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Western Blot (WB) (Mouse Map3k6 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the Map3k6 antibody detected the Map3k6 protein (arrow).)

Rabbit anti-Human, mouse Map3k6 Polyclonal Antibody | anti-Map3k6 antibody

Mouse Map3k6 Antibody (N-term)

Reactivity
Human, mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Map3k6; Polyclonal Antibody; Mouse Map3k6 Antibody (N-term); Mitogen-activated protein kinase kinase kinase 6; Apoptosis signal-regulating kinase 2; Map3k6 {ECO:0000312|MGI:MGI:1855691}; anti-Map3k6 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Map3k6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-252 amino acids from the N-terminal region of mouse Map3k6.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
223-252
Sequence Length
1,291
Applicable Applications for anti-Map3k6 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
MOUSE
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Mouse Map3k6 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the Map3k6 antibody detected the Map3k6 protein (arrow).)

Western Blot (WB) (Mouse Map3k6 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the Map3k6 antibody detected the Map3k6 protein (arrow).)

Western Blot (WB)

(Mouse Map3k6 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the Map3k6 antibody detected the Map3k6 protein (arrow).)

Western Blot (WB) (Mouse Map3k6 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the Map3k6 antibody detected the Map3k6 protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Map3k6 Antibody (N-term) (MBS9208749)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map3k6 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Mouse Map3k6 Antibody (N-term) (MBS9208749)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map3k6 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-Map3k6 antibody
Component of a protein kinase signal transduction cascade. Activates the JNK, but not ERK or p38 kinase pathways.
References
Quina, L.A., et al. J. Neurosci. 29(45):14309-14322(2009)
Iriyama, T., et al. EMBO J. 28(7):843-853(2009)
Eto, N., et al. Am. J. Pathol. 174(4):1553-1563(2009)
Craig, E.A., et al. Dev. Dyn. 237(11):3102-3114(2008)
Breitwieser, W., et al. Genes Dev. 21(16):2069-2082(2007)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
143055
NCBI Official Full Name
Mitogen-activated protein kinase kinase kinase 6
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 6
UniProt Gene Name
Map3k6
UniProt Entry Name
M3K6_MOUSE

Uniprot Description

MEKK6: a member of the STE11 protein kinase family. Activates JNK, but not ERK or p38. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Protein type: EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, STE; STE group; STE11 family

Cellular Component: cytoplasm

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; metal ion binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; magnesium ion binding; kinase activity; ATP binding; protein kinase activity

Biological Process: activation of MAPKK activity; MAPKKK cascade; response to stress; protein amino acid phosphorylation; phosphorylation

Similar Products

Product Notes

The Map3k6 map3k6 (Catalog #AAA9208749) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 223-252. The Mouse Map3k6 Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Map3k6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the Map3k6 map3k6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Map3k6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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