Rat Neutrophil gelatinase-associated lipocalin ELISA Kit | Lcn2 elisa kit

Rat Neutrophil gelatinase-associated lipocalin ELISA Kit

Cat. Num. AAA2884287

• Gene Names: Lcn2; Sip24
• Reactivity: Rat
• Synonyms: Neutrophil gelatinase-associated lipocalin; Rat Neutrophil gelatinase-associated lipocalin ELISA Kit; NGAL; Alpha-2-microglobulin-related protein; Alpha-2U globulin-related protein; Lipocalin-2; p25; Lcn2; Lcn2 elisa kit

• Reactivity: Rat
• Specificity: Recombinant and natural Rat Neutrophil gelatinase-associated lipocalin
• Sequence Length: 198

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.78-50 ng/mL
• Sensitivity: <0.46 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=5.2%
• Inter-assay Precision: Inter-Assay CV: <=7.1%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Lcn2 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18543345
• NCBI GeneID: 170496
• NCBI Accession #: NP_570097.1
• NCBI GenBank Nucleotide #: NM_130741.1
• UniProt Accession #: P30152; Q5HZF1
• Molecular Weight: 22,476 Da
• NCBI Official Full Name: neutrophil gelatinase-associated lipocalin
• NCBI Official Synonym Full Names: lipocalin 2
• NCBI Official Symbol: Lcn2
• NCBI Official Synonym Symbols: Sip24
• NCBI Protein Information: neutrophil gelatinase-associated lipocalin
• UniProt Protein Name: Neutrophil gelatinase-associated lipocalin
• Protein Family: Neutrophil gelatinase-associated lipocalin
• UniProt Gene Name: Lcn2
• UniProt Synonym Gene Names: NGAL
• UniProt Entry Name: NGAL_RAT

NCBI Description

mouse homolog plays a role in IL3 withdrawal-induced apoptosis [RGD, Feb 2006]

Uniprot Description

LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Transporter; Secreted; Secreted, signal peptide

Cellular Component: cytosol; extracellular region; extracellular space

Molecular Function: iron ion binding; protease binding; protein homodimerization activity; transporter activity

Biological Process: cellular response to nutrient levels; innate immune response; ion transport; iron ion homeostasis; positive regulation of cell projection organization and biogenesis; response to bacterium; response to drug; response to herbicide; response to mycotoxin; response to nutrient levels; response to oxidative stress; response to virus; siderophore transport