Western Blot (WB)
(SPB4 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SPB4 antibody detected the SPB4 protein (arrow).)
Rabbit anti-Human SPB4 Polyclonal Antibody | anti-SPB4 antibody
SPB4, CT (SERPINB4, PI11, SCCA2, Serpin B4, Leupin, Peptidase inhibitor 11, Squamous cell carcinoma antigen 2) (APC)
Gene Names
SERPINB4; PI11; SCCA1; SCCA2; LEUPIN; SCCA-2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SPB4; Polyclonal Antibody; CT (SERPINB4; PI11; SCCA2; Serpin B4; Leupin; Peptidase inhibitor 11; Squamous cell carcinoma antigen 2) (APC); SERPINB4; Squamous cell carcinoma antigen 2; anti-SPB4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-SPB4 antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
SPB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-304 amino acids from the C-terminal region of human SPB4.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(SPB4 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SPB4 antibody detected the SPB4 protein (arrow).)
Western Blot (WB)
(SPB4 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SPB4 antibody detected the SPB4 protein (arrow).)
Flow Cytometry (FC/FACS)
(SPB4 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(SPB4 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-SPB4 antibody
SPB4 may act as a protease inhibitor to modulate the host immune response against tumor cells.
Product Categories/Family for anti-SPB4 antibody
References
Murakami, A., et al. Int. J. Oncol. 36(6):1395-1400(2010). Higgins, W.J., et al. J. Biol. Chem. 285(6):3722-3729(2010). Villano, G., et al. Int. J. Mol. Med. 25(1):137-143(2010). Ahmed, S.T., et al. Biochem. Biophys. Res. Commun. 378(4):821-825(2009). Yamane, Y., et al. Int. Arch. Allergy Immunol. 150(1):89-101(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,854 Da
NCBI Official Full Name
serpin B4
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade B (ovalbumin), member 4
NCBI Official Symbol
SERPINB4
NCBI Official Synonym Symbols
PI11; SCCA1; SCCA2; LEUPIN; SCCA-2
NCBI Protein Information
serpin B4; PI-11; peptidase inhibitor 11; SCCA2/SCCA1 fusion protein; squamous cell carcinoma antigen 1; squamous cell carcinoma antigen 2; protease inhibitor (leucine-serpin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 4
UniProt Protein Name
Serpin B4
Protein Family
UniProt Gene Name
SERPINB4
UniProt Synonym Gene Names
PI11; SCCA2; PI-11; SCCA-2
UniProt Entry Name
SPB4_HUMAN
Uniprot Description
SERPINB4: May act as a protease inhibitor to modulate the host immune response against tumor cells. Belongs to the serpin family. Ov-serpin subfamily.
Protein type: Inhibitor
Chromosomal Location of Human Ortholog: 18q21.3
Cellular Component: extracellular space; cytoplasm; intracellular
Molecular Function: serine-type endopeptidase inhibitor activity; enzyme binding; protease binding
Biological Process: regulation of proteolysis; negative regulation of peptidase activity; protection from natural killer cell mediated cytotoxicity
Research Articles on SPB4
Similar Products
Product Notes
The SPB4 serpinb4 (Catalog #AAA6350410) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPB4, CT (SERPINB4, PI11, SCCA2, Serpin B4, Leupin, Peptidase inhibitor 11, Squamous cell carcinoma antigen 2) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPB4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SPB4 serpinb4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPB4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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