Pig Peroxisome proliferator-activated receptor gamma ELISA Kit | PPARG elisa kit

Pig Peroxisome proliferator-activated receptor gamma ELISA Kit

Cat. Num. AAA288311

• Reactivity: Pig
• Synonyms: Peroxisome proliferator-activated receptor gamma; Pig Peroxisome proliferator-activated receptor gamma ELISA Kit; PPARG elisa kit

• Reactivity: Pig
• Specificity: This assay recognizes recombinant and natural pig PPAR-gamma. No significant cross-reactivity or interference was observed.
• Sequence Length: 475

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Detection Range: 0.78 ng/ml -50.0 ng/ml.
• Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of pig PPAR-gamma concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PPARG elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPAR-gamma. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PPAR-gamma and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PPAR-gamma, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PPAR-gamma in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 47523814
• NCBI GeneID: 397671
• NCBI Accession #: NP_999544.1
• NCBI GenBank Nucleotide #: NM_214379.1
• UniProt Accession #: O62807; O77815; Q6L9M2
• Molecular Weight: 54,474 Da
• NCBI Official Full Name: peroxisome proliferator-activated receptor gamma
• NCBI Official Symbol: PPARG
• NCBI Protein Information: peroxisome proliferator-activated receptor gamma
• UniProt Protein Name: Peroxisome proliferator-activated receptor gamma
• Protein Family: Peroxisome proliferator-activated receptor
• UniProt Gene Name: PPARG
• UniProt Synonym Gene Names: NR1C3; PPAR-gamma
• UniProt Entry Name: PPARG_PIG

Uniprot Description

Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels.