Western Blot (WB)
(Western Blot analysis using MBS643049 in 293 cell line lysates (35ug/lane). This demonstrates the H1FX antibody detected the H1FX protein (arrow).)
Rabbit anti-Human H1FX Polyclonal Antibody | anti-H1FX antibody
H1FX, CT (H1FX, Histone H1x) (PE)
Gene Names
H1FX; H1X
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
H1FX; Polyclonal Antibody; CT (H1FX; Histone H1x) (PE); Histone H1x; anti-H1FX antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-H1FX antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
H1FX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-167 amino acids from the C-terminal region of human H1FX.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis using MBS643049 in 293 cell line lysates (35ug/lane). This demonstrates the H1FX antibody detected the H1FX protein (arrow).)
Western Blot (WB)
(Western Blot analysis using MBS643049 in 293 cell line lysates (35ug/lane). This demonstrates the H1FX antibody detected the H1FX protein (arrow).)
Related Product Information for anti-H1FX antibody
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H1 family.
Product Categories/Family for anti-H1FX antibody
References
Warneboldt, J., et al. BMC Cancer 8, 388 (2008): Stoldt, S., et al. Biol. Cell 99(10):541-552(2007). Takata, H., et al. FEBS Lett. 581(20):3783-3788(2007). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,487 Da
NCBI Official Full Name
histone H1x
NCBI Official Synonym Full Names
H1 histone family, member X
NCBI Official Symbol
H1FX
NCBI Official Synonym Symbols
H1X
NCBI Protein Information
histone H1x
UniProt Protein Name
Histone H1x
Protein Family
UniProt Gene Name
H1FX
UniProt Entry Name
H1X_HUMAN
NCBI Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H1 family. [provided by RefSeq, Jul 2008]
Uniprot Description
H1X: Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. Belongs to the histone H1/H5 family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 3q21.3
Cellular Component: nucleolus; nucleosome; nucleus
Molecular Function: DNA binding
Biological Process: nucleosome assembly
Research Articles on H1FX
Similar Products
Product Notes
The H1FX h1fx (Catalog #AAA6305217) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The H1FX, CT (H1FX, Histone H1x) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's H1FX can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the H1FX h1fx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "H1FX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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