Mouse Axin-1 ELISA Kit | Axin1 elisa kit

Mouse Axin-1 ELISA Kit

Cat. Num. AAA288281

• Gene Names: Axin1; Fu; Kb; Ki; Axin; fused; kinky; knobbly; AI316800
• Reactivity: Mouse
• Synonyms: Axin-1; Mouse Axin-1 ELISA Kit; Axin1 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse Axin-1. No significant cross-reactivity or interference was observed.
• Sequence Length: 826

• Assay Type: Sandwich
• Detection Range: 78-5000 pg/ml
• Sensitivity: 39.8 pg/mL
• Intra-Assay CV: <=6.3%
• Inter-Assay CV: <=8.2%
• Recovery: 84%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Axin1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Axin-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Axin-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Axin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Axin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 227430346
• NCBI GeneID: 12005
• NCBI Accession #: NP_033863.2
• NCBI GenBank Nucleotide #: NM_009733.2
• UniProt Accession #: O35625
• Molecular Weight: 92,418 Da
• NCBI Official Full Name: axin-1 isoform 2
• NCBI Official Synonym Full Names: axin 1
• NCBI Official Symbol: Axin1
• NCBI Official Synonym Symbols: Fu; Kb; Ki; Axin; fused; kinky; knobbly; AI316800
• NCBI Protein Information: axin-1
• UniProt Protein Name: Axin-1
• Protein Family: Axin
• UniProt Gene Name: Axin1
• UniProt Synonym Gene Names: Axin; Fu
• UniProt Entry Name: AXIN1_MOUSE

Uniprot Description

axin 1: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin-1 functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Wild-type axin 1 can induce apoptosis in hepatocellular and colorectal cancer cells. Is downregulated during progression of esophageal squamous cell carcinoma. Mutation of the axin-1 gene is associated with hepatocellular carcinoma, anaplastic thyroid cancer, medulloblastoma and colorectal cancer. May have a role in oncogenesis in Hodgkin lymphoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways.

Protein type: Tumor suppressor; Adaptor/scaffold

Cellular Component: beta-catenin destruction complex; cell cortex; cytoplasm; cytoplasmic membrane-bound vesicle; cytoplasmic microtubule; cytoplasmic vesicle; Golgi apparatus; lateral plasma membrane; membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; postsynaptic density; protein complex

Molecular Function: beta-catenin binding; enzyme binding; GTPase activator activity; identical protein binding; p53 binding; protein binding; protein C-terminus binding; protein complex scaffold; protein domain specific binding; protein homodimerization activity; protein kinase binding; protein self-association; receptor binding; receptor signaling complex scaffold activity; signal transducer activity; SMAD binding; ubiquitin protein ligase binding

Biological Process: activation of JNK activity; activation of protein kinase activity; adult walking behavior; apoptosis; axial mesoderm development; axial mesoderm formation; cell death; cellular protein complex assembly; cytoplasmic microtubule organization and biogenesis; determination of left/right symmetry; dorsal/ventral axis specification; dorsal/ventral pattern formation; embryonic eye morphogenesis; erythrocyte homeostasis; forebrain anterior/posterior pattern formation; hemoglobin metabolic process; in utero embryonic development; multicellular organismal development; muscle cell development; negative regulation of fat cell differentiation; negative regulation of protein metabolic process; negative regulation of Wnt receptor signaling pathway; neurological system process; nucleocytoplasmic transport; olfactory placode formation; optic placode formation; positive regulation of JNK activity; positive regulation of JNK cascade; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein amino acid phosphorylation; positive regulation of protein catabolic process; positive regulation of protein kinase activity; positive regulation of protein ubiquitination; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; positive regulation of ubiquitin-protein ligase activity; protein catabolic process; protein homooligomerization; protein polyubiquitination; regulation of protein amino acid phosphorylation; sensory perception of sound; signal transduction; Wnt receptor signaling pathway; Wnt receptor signaling pathway involved in forebrain neuron fate commitment; Wnt receptor signaling pathway through beta-catenin