Pig Chymotrypsin-like elastase family member 1 ELISA Kit | CELA1 elisa kit

Pig Chymotrypsin-like elastase family member 1 ELISA Kit

Cat. Num. AAA288129

• Gene Names: CELA1; ELA1; ELS1; Elastase-1
• Reactivity: Pig
• Synonyms: Chymotrypsin-like elastase family member 1; Pig Chymotrypsin-like elastase family member 1 ELISA Kit; CELA1 elisa kit

• Reactivity: Pig
• Specificity: This assay recognizes recombinant and natural pig Chymotrypsin-like elastase family member 1. No significant cross-reactivity or interference was observed.
• Sequence Length: 258

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Detection Range: 78.0 pg/ml -5000 pg/ml.
• Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of pig Chymotrypsin-like elastase family member 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CELA1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chymotrypsin-like elastase family member 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Chymotrypsin-like elastase family member 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Chymotrypsin-like elastase family member 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Chymotrypsin-like elastase family member 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 50979297
• NCBI GeneID: 396766
• NCBI Accession #: NP_998988.1
• NCBI GenBank Nucleotide #: NM_213823.1
• UniProt Accession #: P00772; Q29625
• Molecular Weight: 28,821 Da
• NCBI Official Full Name: chymotrypsin-like elastase family member 1 preproprotein
• NCBI Official Symbol: CELA1
• NCBI Official Synonym Symbols: ELA1; ELS1; Elastase-1
• NCBI Protein Information: chymotrypsin-like elastase family member 1
• UniProt Protein Name: Chymotrypsin-like elastase family member 1
• Protein Family: Chymotrypsin-like elastase family
• UniProt Gene Name: CELA1
• UniProt Synonym Gene Names: ELA1
• UniProt Entry Name: CELA1_PIG

Uniprot Description

CELA1: Acts upon elastin

Protein type: Secreted; EC 3.4.21.36; Secreted, signal peptide; Protease

Cellular Component: extracellular space

Molecular Function: metal ion binding; protein binding; serine-type endopeptidase activity

Biological Process: exocrine pancreas development; inflammatory response; multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; positive regulation of angiogenesis; positive regulation of transcription from RNA polymerase II promoter; post-embryonic development; proteolysis; regulation of cell differentiation; regulation of cell proliferation; tissue remodeling; Wnt receptor signaling pathway