Mouse Angiotensin-converting enzyme ELISA Kit | Ace elisa kit

Mouse Angiotensin-converting enzyme ELISA Kit

Cat. Num. AAA2880978

• Gene Names: Ace; CD143; AW208573
• Reactivity: Mouse
• Synonyms: Angiotensin-converting enzyme; Mouse Angiotensin-converting enzyme ELISA Kit; ACE; Dipeptidyl carboxypeptidase I; Kininase II; Ace; Dcp1; 3.2.1.-; CD143; Ace elisa kit

• Reactivity: Mouse
• Sequence Length: 732

• Assay Type: Sandwich
• Detection Range: 78-5000 pg/mL
• Sensitivity: 39 pg/mL
• Intra-Assay CV: <=5.4%
• Inter-Assay CV: <=9.1%
• Recovery: 98%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Related Product Information for Ace elisa kit

Intended Uses||This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 33468873
• NCBI GeneID: 11421
• NCBI Accession #: NP_033728.1
• NCBI GenBank Nucleotide #: NM_009598.2
• UniProt Accession #: P09470; P22967; Q6GTS2
• Molecular Weight: 84,047 Da
• NCBI Official Full Name: angiotensin-converting enzyme isoform 2
• NCBI Official Synonym Full Names: angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
• NCBI Official Symbol: Ace
• NCBI Official Synonym Symbols: CD143; AW208573
• NCBI Protein Information: angiotensin-converting enzyme
• UniProt Protein Name: Angiotensin-converting enzyme
• Protein Family: Acetylcholinesterase
• UniProt Gene Name: Ace
• UniProt Synonym Gene Names: Dcp1; ACE
• UniProt Entry Name: ACE_MOUSE

Uniprot Description

ACE: Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. Genetic variations in ACE may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in ACE are a cause of renal tubular dysgenesis (RTD). RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Genetic variations in ACE are associated with susceptibility to microvascular complications of diabetes type 3 (MVCD3). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Defects in ACE are a cause of susceptibility to intracerebral hemorrhage (ICH). A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Belongs to the peptidase M2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.15.1; Membrane protein, integral

Cellular Component: basal plasma membrane; brush border membrane; cytoplasm; endosome; external side of plasma membrane; extracellular region; extracellular space; integral to membrane; lysosome; membrane; plasma membrane; vesicle

Molecular Function: actin binding; amylase activity; bradykinin receptor binding; carboxypeptidase activity; chloride ion binding; drug binding; endopeptidase activity; exopeptidase activity; fucosidase activity; galactosidase activity; glucosidase activity; hexosaminidase activity; hydrolase activity; lytic transglycosylase activity; mannosidase activity; mannosyl-oligosaccharide mannosidase activity; metal ion binding; metallopeptidase activity; mitogen-activated protein kinase binding; mitogen-activated protein kinase kinase binding; peptidase activity; peptidyl-dipeptidase activity; protein binding; trehalase activity; tripeptidyl-peptidase activity; zinc ion binding

Biological Process: alveolus development; arachidonic acid secretion; beta-amyloid metabolic process; eating behavior; heart contraction; hormone catabolic process; kidney development; negative regulation of protein binding; neutrophil mediated immunity; organ regeneration; peptide catabolic process; peptide metabolic process; positive regulation of apoptosis; positive regulation of inflammatory response; positive regulation of neurogenesis; positive regulation of protein binding; positive regulation of systemic arterial blood pressure; proteolysis; regulation of angiotensin metabolic process; regulation of blood pressure; regulation of smooth muscle cell migration; regulation of systemic arterial blood pressure by renin-angiotensin; response to lipopolysaccharide; sensory perception of pain; spermatogenesis; vasoconstriction