Mouse CD143 ELISA Kit | ACE elisa kit

Mouse CD143 ELISA Kit

Cat. Num. AAA824479

• Gene Names: Ace; CD143; AW208573
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: CD143; Mouse CD143 ELISA Kit; DCP; DCP1; Angiotensin-converting enzyme; ACE; Dipeptidyl carboxypeptidase I; Kininase II; ACE elisa kit

• Reactivity: Mouse
• Specificity: The Mouse CD143 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD143 proteins within the range of 93.8 pg/ml - 6000 pg/ml.
• Sequence Length: 732

• Applicable Applications for ACE elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernatants, Serum, Plasma.
• Sensitivity: 10 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Related Product Information for ACE elisa kit

Principle of the Assay||TheMouse CD143 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD143 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CD143 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD143 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD143 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD143 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm.

NCBI and Uniprot Product Information

• NCBI GI #: 33468873
• NCBI GeneID: 11421
• NCBI Accession #: NP_033728.1
• NCBI GenBank Nucleotide #: NM_009598.2
• UniProt Accession #: P09470; P22967; Q6GTS2
• Molecular Weight: 84,047 Da
• NCBI Official Full Name: angiotensin-converting enzyme isoform 2
• NCBI Official Synonym Full Names: angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
• NCBI Official Symbol: Ace
• NCBI Official Synonym Symbols: CD143; AW208573
• NCBI Protein Information: angiotensin-converting enzyme; kininase II; dipeptidyl peptidase; dipeptidyl carboxypeptidase I
• UniProt Protein Name: Angiotensin-converting enzyme
• Protein Family: Acetylcholinesterase
• UniProt Gene Name: Ace
• UniProt Synonym Gene Names: Dcp1; ACE
• UniProt Entry Name: ACE_MOUSE

Uniprot Description

ACE: Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. Genetic variations in ACE may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in ACE are a cause of renal tubular dysgenesis (RTD). RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Genetic variations in ACE are associated with susceptibility to microvascular complications of diabetes type 3 (MVCD3). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Defects in ACE are a cause of susceptibility to intracerebral hemorrhage (ICH). A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Belongs to the peptidase M2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.15.1; Membrane protein, integral

Cellular Component: extracellular space; membrane; lysosome; brush border membrane; cytoplasm; basal plasma membrane; integral to membrane; plasma membrane; extracellular region; vesicle; endosome; external side of plasma membrane

Molecular Function: carboxypeptidase activity; zinc ion binding; metallopeptidase activity; mitogen-activated protein kinase kinase binding; metal ion binding; mannosyl-oligosaccharide mannosidase activity; drug binding; actin binding; glucosidase activity; endopeptidase activity; mitogen-activated protein kinase binding; amylase activity; chloride ion binding; tripeptidyl-peptidase activity; peptidyl-dipeptidase activity; hydrolase activity; trehalase activity; peptidase activity; protein binding; fucosidase activity; galactosidase activity; lytic transglycosylase activity; hexosaminidase activity; bradykinin receptor binding; exopeptidase activity; mannosidase activity

Biological Process: positive regulation of protein binding; positive regulation of apoptosis; neutrophil mediated immunity; positive regulation of systemic arterial blood pressure; regulation of angiotensin metabolic process; response to lipopolysaccharide; proteolysis; sensory perception of pain; regulation of smooth muscle cell migration; peptide metabolic process; regulation of blood pressure; beta-amyloid metabolic process; peptide catabolic process; kidney development; negative regulation of protein binding; vasoconstriction; positive regulation of neurogenesis; organ regeneration; eating behavior; arachidonic acid secretion; regulation of systemic arterial blood pressure by renin-angiotensin; heart contraction; spermatogenesis; hormone catabolic process; alveolus development; positive regulation of inflammatory response