Typical Testing Data/Standard Curve (for reference only)
Dog Granulocyte colony-stimulating factor ELISA Kit | CSF3 elisa kit
Dog Granulocyte colony-stimulating factor ELISA Kit
Reactivity
Dog
Synonyms
Granulocyte colony-stimulating factor; Dog Granulocyte colony-stimulating factor ELISA Kit; G-CSF; CSF3; CSF3 elisa kit
Reactivity
Dog
Specificity
This assay recognizes recombinant and natural dogG-CSF. No significant cross-reactivity or interference was observed.
Sequence Length
175
Samples
Serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Detection Range
15.6pg/mL -1000pg/mL.
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CSF3 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination ofdogG-CSFconcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to G-CSF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forG-CSF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain G-CSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of G-CSF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to G-CSF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forG-CSF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain G-CSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of G-CSF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
18,858 Da
NCBI Official Full Name
Granulocyte colony-stimulating factor
UniProt Protein Name
Granulocyte colony-stimulating factor
Protein Family
UniProt Gene Name
CSF3
UniProt Synonym Gene Names
G-CSF
UniProt Entry Name
CSF3_CANLF
Uniprot Description
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
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Product Notes
The Dog CSF3 csf3 (Catalog #AAA2880747) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2880747 ELISA Kit recognizes Dog CSF3. It is sometimes possible for the material contained within the vial of "Granulocyte colony-stimulating factor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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