Typical Testing Data/Standard Curve (for reference only)
Rat Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit | GCSF elisa kit
Rat Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit
Gene Names
Csf3; Gcsf
Reactivity
Rat
Synonyms
Colony Stimulating Factor 3; Granulocyte (GCSF); Rat Colony Stimulating Factor 3; Granulocyte (GCSF) ELISA Kit; CSF3; G-CSF; Granulocyte Colony Stimulating Factor; Pluripoietin; Filgrastim; Lenograstim; GCSF elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of GCSF. No significant cross-reactivity or interference between GCSF and analogues was observed.
Sequence Length
214
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Type
Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
5.3pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GCSF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GCSF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GCSF elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GCSF in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GCSF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GCSF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GCSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GCSF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GCSF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GCSF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GCSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GCSF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
23,660 Da
NCBI Official Full Name
Granulocyte colony stimulating factor
NCBI Official Synonym Full Names
colony stimulating factor 3
NCBI Official Symbol
Csf3
NCBI Official Synonym Symbols
Gcsf
NCBI Protein Information
granulocyte colony-stimulating factor
UniProt Protein Name
Granulocyte colony stimulating factor
UniProt Gene Name
Csf3
NCBI Description
putative hematopoetic growth factor for neutrophils; used as a treatment for cyclic hematopoesis in humans and dogs [RGD, Feb 2006]
Research Articles on GCSF
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Product Notes
The Rat GCSF csf3 (Catalog #AAA453665) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA453665 ELISA Kit recognizes Rat GCSF. It is sometimes possible for the material contained within the vial of "Colony Stimulating Factor 3, Granulocyte (GCSF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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