Human Ubiquitin-conjugating enzyme E2 D4 (UBE2D4) ELISA Kit | UBE2D4 elisa kit
Human Ubiquitin-conjugating enzyme E2 D4 (UBE2D4) ELISA Kit
Gene Names
UBE2D4; HBUCE1
Reactivity
Human
Synonyms
Ubiquitin-conjugating enzyme E2 D4 (UBE2D4); Human Ubiquitin-conjugating enzyme E2 D4 (UBE2D4) ELISA Kit; HBUCE1; ubiquitin-conjugating enzyme E2D 4; ubiquitin-conjugating enzyme HBUCE1; UBE2D4 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human UBE2D4. No significant cross-reactivity or interference between Human UBE2D4 and analogues was observed.
Sequence Length
147
Assay Type
Quantitive Sandwich
Samples
Serum, Plasma, Other Biological Fluids
Detection Wavelength
450 nm
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%)
= SD/meanX100
Intra-Assay
CV
Inter-Assay
CV
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for UBE2D4 elisa kit
This assay employs a two-site sandwich ELISA to quantitate UBE2D4 in samples. An antibody specific for UBE2D4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UBE2D4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE2D4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2D4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,649 Da
NCBI Official Full Name
ubiquitin-conjugating enzyme E2 D4
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 D4 (putative)
NCBI Official Symbol
UBE2D4
NCBI Official Synonym Symbols
HBUCE1
NCBI Protein Information
ubiquitin-conjugating enzyme E2 D4
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 D4
Protein Family
UniProt Gene Name
UBE2D4
UniProt Synonym Gene Names
UBCH5D
Uniprot Description
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.
Research Articles on UBE2D4
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Product Notes
The Human UBE2D4 ube2d4 (Catalog #AAA287855) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA287855 ELISA Kit recognizes Human UBE2D4. It is sometimes possible for the material contained within the vial of "Ubiquitin-conjugating enzyme E2 D4 (UBE2D4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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