Mouse IDS ELISA Kit | IDS elisa kit

Mouse IDS ELISA Kit

Cat. Num. AAA8248442

• Gene Names: Ids; AW214631
• Reactivity: Mouse
• Synonyms: IDS; Mouse IDS ELISA Kit; SIDS; Iduronate 2-sulfatase; Alpha-L-iduronate sulfate sulfatase; Idursulfase; IDS elisa kit

• Reactivity: Mouse
• Specificity: The Mouse IDS ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse IDS proteins within the range of 156 pg/ml - 10000 pg/ml.
• Sequence Length: 552

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <15 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IDS elisa kit

Principle of the Assay: The Mouse IDS ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IDS in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse IDS coated on a 96-well plate. Standards and samples are pipetted into the wells and IDS present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IDS antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IDS bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Iduronate 2-sulfatase (IDS) is a sulfatase enzyme associated with Hunter syndrome. It encodes a member of the sulfatase family of proteins. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

NCBI and Uniprot Product Information

• NCBI GI #: 84781812
• NCBI GeneID: 15931
• NCBI Accession #: NP_034628.2
• NCBI GenBank Nucleotide #: NM_010498.3
• UniProt Accession #: Q08890; Q32KI7; Q3TM30
• Molecular Weight: 62,186 Da
• NCBI Official Full Name: iduronate 2-sulfatase
• NCBI Official Synonym Full Names: iduronate 2-sulfatase
• NCBI Official Symbol: Ids
• NCBI Official Synonym Symbols: AW214631
• NCBI Protein Information: iduronate 2-sulfatase
• UniProt Protein Name: Iduronate 2-sulfatase
• Protein Family: Iduronate 2-sulfatase
• UniProt Gene Name: Ids
• UniProt Entry Name: IDS_MOUSE

Uniprot Description

IDS: Required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Defects in IDS are the cause of mucopolysaccharidosis type 2 (MPS2); also known as Hunter syndrome. MPS2 is an X-linked lysosomal storage disease characterized by intracellular accumulation of heparan sulfate and dermatan sulfate and their excretion in urine. Most children with MPS2 have a severe form with early somatic abnormalities including skeletal deformities, hepatosplenomegaly, and progressive cardiopulmonary deterioration. A prominent feature is neurological damage that presents as developmental delay and hyperactivity but progresses to mental retardation and dementia. They die before 15 years of age, usually as a result of obstructive airway disease or cardiac failure. In contrast, those with a mild form of MPS2 may survive into adulthood, with attenuated somatic complications and often without mental retardation. Belongs to the sulfatase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.6.13; Glycan Metabolism - glycosaminoglycan degradation; Hydrolase

Molecular Function: protein binding; sulfuric ester hydrolase activity