Western Blot (WB)
(Western blot of chicken brain tissue incubated with CLC4 Antibody (C-term) . Data courtesy of Emily McMains, Louisiana State University.)
Rabbit anti-Human CLC4 Polyclonal Antibody
CLC4, CT (H(+)/Cl(-) Exchange Transporter 4, Chloride Channel Protein 4, Chloride Transporter ClC-4, ClC-4, CLCN4)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Western Blot (WB)
(Western blot of chicken brain tissue incubated with CLC4 Antibody (C-term) . Data courtesy of Emily McMains, Louisiana State University.)
Western Blot (WB)
(Western blot of chicken brain tissue incubated with CLC4 Antibody (C-term) . Data courtesy of Emily McMains, Louisiana State University.)
Immunofluorescence (IF)
(Immunofluorescence image of cultured chick retinal amacrine (neuronal) cells labeled with CLC4 Antibody (C-term) . Data courtesy of Emily McMains, Louisiana State University.)
Immunofluorescence (IF)
(Immunofluorescence image of cultured chick retinal amacrine (neuronal) cells labeled with CLC4 Antibody (C-term) . Data courtesy of Emily McMains, Louisiana State University.)
Testing Data
(Retinae were collected from adult White Leghorn chicken and fixed in the eyecup for two hours in 4% Paraformaldehyde (in PBS). Retinae were then removed from the eyecups and incubated in 30% sucrose (in PBS) overnight. Retinal tissue was embedded and frozen in OCT compound and cut into ~15um sections on a cryotome. Sections were blocked in 5% normal goat serum (in 1%BSA/.1%saponin PBS) for one hour and then incubated at RT with 1:250 (1%BSA/.1% saponin PBS) ClC4 antibody for 1 hour. Sections were then washed in PBS (3X10minutes) and then treated with secondary antibody (1:500 Cy3) for one hour. After another PBS wash series, sections were coverslipped and antibody labeling was visualized at 20X with a Leica upright microscope using a TRITC filter set and Xenon lamp illumination. (Crousillac et al, 2003))
Testing Data
(Retinae were collected from adult White Leghorn chicken and fixed in the eyecup for two hours in 4% Paraformaldehyde (in PBS). Retinae were then removed from the eyecups and incubated in 30% sucrose (in PBS) overnight. Retinal tissue was embedded and frozen in OCT compound and cut into ~15um sections on a cryotome. Sections were blocked in 5% normal goat serum (in 1%BSA/.1%saponin PBS) for one hour and then incubated at RT with 1:250 (1%BSA/.1% saponin PBS) ClC4 antibody for 1 hour. Sections were then washed in PBS (3X10minutes) and then treated with secondary antibody (1:500 Cy3) for one hour. After another PBS wash series, sections were coverslipped and antibody labeling was visualized at 20X with a Leica upright microscope using a TRITC filter set and Xenon lamp illumination. (Crousillac et al, 2003))
NCBI and Uniprot Product Information
NCBI Description
The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. Chloride channel 4 has an evolutionary conserved CpG island and is conserved in both mouse and hamster. This gene is mapped in close proximity to APXL (Apical protein Xenopus laevis-like) and OA1 (Ocular albinism type I), which are both located on the human X chromosome at band p22.3. The physiological role of chloride channel 4 remains unknown but may contribute to the pathogenesis of neuronal disorders. Alternate splicing results in two transcript variants that encode different proteins. [provided by RefSeq, Mar 2012]
Uniprot Description
CLCN4: Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Belongs to the chloride channel (TC 2.A.49) family. ClC-4/CLCN4 subfamily.
Protein type: Membrane protein, multi-pass; Transporter, ion channel; Transporter; Membrane protein, integral
Chromosomal Location of Human Ortholog: Xp22.3
Cellular Component: early endosome membrane; late endosome membrane; integral to membrane; endosome membrane
Molecular Function: chloride channel activity; voltage-gated chloride channel activity; ATP binding; antiporter activity
Biological Process: transport; chloride transport; transmembrane transport
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Product Notes
The CLC4 (Catalog #AAA627739) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CLC4, CT (H(+)/Cl(-) Exchange Transporter 4, Chloride Channel Protein 4, Chloride Transporter ClC-4, ClC-4, CLCN4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CLC4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot, and Immunofluorescence. Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500. Researchers should empirically determine the suitability of the CLC4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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