Human Cyclin-dependent kinase 8 (CDK8) ELISA Kit | CDK8 elisa kit

Human Cyclin-dependent kinase 8 (CDK8) ELISA Kit

Cat. Num. AAA287363

• Gene Names: CDK8; K35
• Reactivity: Human
• Synonyms: Cyclin-dependent kinase 8 (CDK8); Human Cyclin-dependent kinase 8 (CDK8) ELISA Kit; K35; MGC126074; MGC126075; CDK8 protein kinase; cell division protein kinase 8; protein kinase K35; CDK8 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human CDK8. No significant cross-reactivity or interference between Human CDK8 and analogues was observed.
• Sequence Length: 464

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for CDK8 elisa kit

This assay employs a two-site sandwich ELISA to quantitate CDK8 in samples. An antibody specific for CDK8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CDK8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CDK8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CDK8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4502745
• NCBI GeneID: 1024
• NCBI Accession #: NP_001251.1
• NCBI GenBank Nucleotide #: NM_001260.2
• UniProt Accession #: P49336; Q5VUF3; Q6ISB5
• Molecular Weight: 53,156 Da
• NCBI Official Full Name: cyclin-dependent kinase 8 isoform 1
• NCBI Official Synonym Full Names: cyclin dependent kinase 8
• NCBI Official Symbol: CDK8
• NCBI Official Synonym Symbols: K35
• NCBI Protein Information: cyclin-dependent kinase 8
• UniProt Protein Name: Cyclin-dependent kinase 8
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK8

NCBI Description

This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Uniprot Description

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.