Immunohistochemistry (IHC)
(Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit ITPR1 Polyclonal Antibody | anti-ITPR1 antibody
ITPR1, ID (Inositol 1,4,5-trisphosphate Receptor Type 1, IP3 Receptor Isoform 1, IP3R 1, InsP3R1, Type 1 Inositol 1,4,5-trisphosphate Receptor, Type 1 InsP3 Receptor, INSP3R1)
Purified by immunoaffinity chromatography
Purified by immunoaffinity chromatography
Dilution: Immunofluorescence: 1:100-1:500
ELISA: 1:5000
Immunohistochemistry (Paraffin): 10ug/ml
Immunohistochemistry (IHC)
(Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunofluorescence (IF)
(Immunofluorescence analysis of COS7 cells, using InsP3R1. The picture on the right is treated with the synthesized peptide.)
Immunofluorescence (IF)
(Immunofluorescence analysis of COS7 cells, using InsP3R1. The picture on the right is treated with the synthesized peptide.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an intracellular receptor for inositol 1,4,5-trisphosphate. Upon stimulation by inositol 1,4,5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Nov 2009]
Uniprot Description
Function: Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways
By similarity.
Subunit structure: Homotetramer. Interacts with TRPC4. The PPXXF motif binds HOM1, HOM2 and HOM3. Interacts with RYR1, RYR2, ITPR1, SHANK1 and SHANK3. Interacts with ERP44 in a pH-, redox state- and calcium-dependent manner which results in the inhibition the calcium channel activity. The strength of this interaction inversely correlates with calcium concentration. Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1. Interacts with AHCYL1
By similarity. Interacts with MRVI1 and CABP1 (via N-terminus). Ref.7 Ref.8 Ref.9 Ref.11
Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue specificity: Widely expressed.
Domain: The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.
Post-translational modification: Phosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels
By similarity. Ref.2Phosphorylated on tyrosine residues. Ref.2Ubiquitination at multiple lysines targets ITPR1 for proteasomal degradation. Approximately 40% of the ITPR1-associated ubiquitin is monoubiquitin, and polyubiquitins are both 'Lys-48'- and 'Lys-63'-linked
By similarity.
Involvement in disease: Spinocerebellar ataxia 15 (SCA15) [MIM:606658]: Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is an autosomal dominant cerebellar ataxia (ADCA). It is very slow progressing form with a wide range of onset, ranging from childhood to adult. Most patients remain ambulatory.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.12 Ref.16Spinocerebellar ataxia 29 (SCA29) [MIM:117360]: An autosomal dominant, congenital spinocerebellar ataxia characterized by early motor delay, hypotonia and mild cognitive delay. Affected individuals develop a very slowly progressive or non-progressive gait and limb ataxia associated with cerebellar atrophy on brain imaging. Additional variable features include nystagmus, dysarthria, and tremor.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.17
Miscellaneous: Calcium appears to inhibit ligand binding to the receptor, most probably by interacting with a distinct calcium-binding protein which then inhibits the receptor.
Sequence similarities: Belongs to the InsP3 receptor family.Contains 5 MIR domains.
Caution: Alternative splice sites (AA 1053-1054) represent a non-canonical GA-AG donor-acceptor pair, but are well-supported by all available human transcripts, and by homologous transcripts in mouse, rat and cow.
Research Articles on ITPR1
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Product Notes
The ITPR1 itpr1 (Catalog #AAA645916) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ITPR1, ID (Inositol 1,4,5-trisphosphate Receptor Type 1, IP3 Receptor Isoform 1, IP3R 1, InsP3R1, Type 1 Inositol 1,4,5-trisphosphate Receptor, Type 1 InsP3 Receptor, INSP3R1) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ITPR1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, ELISA, Immunohistochemistry. Dilution: Immunofluorescence: 1:100-1:500 ELISA: 1:5000 Immunohistochemistry (Paraffin): 10ug/ml. Researchers should empirically determine the suitability of the ITPR1 itpr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ITPR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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