Rat DNA mismatch repair protein Mlh1 ELISA Kit | MLH1 elisa kit
Rat DNA mismatch repair protein Mlh1 (MLH1) ELISA Kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MLH1 in Rat serum, plasma, tissue homogenates. An antibody specific for MLH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MLH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MLH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MLH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
may be involved in DNA mismatch repair [RGD, Feb 2006]
Uniprot Description
MLH1: a protein involved in the repair of mismatches in DNA. Binds PMS2 or MLH1 and MLH3. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50- MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with MBD4. Interacts with EXO1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2; Turcot syndrome, an autosomal dominant disorder characterized by malignant tumors of the brain; Muir-Torre syndrome (MTS), a rare autosomal dominant disorder characterized by sebaceous neoplasms and visceral malignancy; lobular carcinoma in situ; endometrial cancer; and non-polyposis colorectal cancer.
Protein type: Cell cycle regulation; DNA repair, damage; Tumor suppressor
Chromosomal Location of Human Ortholog: 8q32
Cellular Component: chiasma; chromosome; condensed chromosome; condensed nuclear chromosome; male germ cell nucleus; membrane; MutLalpha complex; nucleoplasm; nucleus; synaptonemal complex
Molecular Function: ATP binding; ATPase activity; chromatin binding; guanine/thymine mispair binding; mismatched DNA binding; MutSalpha complex binding; single-stranded DNA binding
Biological Process: cellular response to DNA damage stimulus; DNA damage response, signal transduction resulting in induction of apoptosis; DNA repair; double-strand break repair via nonhomologous end joining; female meiosis chromosome segregation; homologous chromosome segregation; isotype switching; male meiosis chromosome segregation; male meiotic nuclear division; meiotic cell cycle; meiotic chromosome segregation; meiotic metaphase I plate congression; meiotic spindle midzone assembly; meiotic telomere clustering; mismatch repair; negative regulation of mitotic recombination; oogenesis; poly(A) tail shortening; positive regulation of isotype switching to IgA isotypes; positive regulation of isotype switching to IgG isotypes; reciprocal meiotic recombination; resolution of meiotic recombination intermediates; response to drug; response to hypoxia; response to toxin; somatic hypermutation of immunoglobulin genes; somatic recombination of immunoglobulin gene segments; somatic recombination of immunoglobulin genes during immune response; spermatogenesis; synapsis
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Product Notes
The Rat MLH1 mlh1 (Catalog #AAA284986) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA284986 ELISA Kit recognizes Rat MLH1. It is sometimes possible for the material contained within the vial of "DNA mismatch repair protein Mlh1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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