Mouse anti-Human MLH1 Monoclonal Antibody | anti-MLH1 antibody

MLH1 Monoclonal Antibody

Cat. Num. AAA2563566

• Gene Names: MLH1; FCC2; COCA2; HNPCC; hMLH1; HNPCC2
• Reactivity: Human
• Applications: Immunohistochemistry, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: MLH1; Monoclonal Antibody; MLH1 Monoclonal Antibody; COCA 2; COCA2; DNA mismatch repair protein Mlh1; FCC 2; FCC2; hMLH 1; hMLH1; HNPCC 2; HNPCC; HNPCC2; MGC5172; MLH 1; MutL homolog 1 (E. coli); MutL homolog 1; MutL homolog 1 colon cancer nonpolyposis type 2; colon cancer; nonpolyposis type 2 (E. coli); MutL protein homolog 1; MutL; E. coli; homolog of; 1; anti-MLH1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: LE0168
• Purity/Purification: Affinity purification
• Form/Format: PBS with 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 756

• Applicable Applications for anti-MLH1 antibody: Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC)
• Application Notes: IHC: 1:100-1:200
• Immunogen: Full length protein from eukaryotic expression system.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20?. Avoid freeze/ thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of huaman colon cancer using MLH1 Monoclonal Antibody dilution of 1:200.)

Related Product Information for anti-MLH1 antibody

Mismatch repair gene hMLH1 is a ubiquitous gene encoding the mismatch repair protein (MMR) known as MutL protein homolog 1 (MLH1). MLH1 is utilized by normal proliferating cells to repair point mutations that may occur during DNA replication. Antibodies to MLH1 are a useful aid for classification of colorectal cancer.

Product Categories/Family for anti-MLH1 antibody

Cancer, Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 4557757
• NCBI GeneID: 4292
• NCBI Accession #: NP_000240.1
• NCBI GenBank Nucleotide #: NP_000240.1
• UniProt Accession #: P40692
• NCBI Official Full Name: DNA mismatch repair protein Mlh1 isoform 1
• NCBI Official Synonym Full Names: mutL homolog 1
• NCBI Official Symbol: MLH1
• NCBI Official Synonym Symbols: FCC2; COCA2; HNPCC; hMLH1; HNPCC2
• NCBI Protein Information: DNA mismatch repair protein Mlh1
• UniProt Protein Name: DNA mismatch repair protein Mlh1
• Protein Family: Putative MutL protein
• UniProt Gene Name: MLH1
• UniProt Synonym Gene Names: COCA2
• UniProt Entry Name: MLH1_HUMAN

NCBI Description

The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017]

Uniprot Description

MLH1: a protein involved in the repair of mismatches in DNA. Binds PMS2 or MLH1 and MLH3. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50- MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with MBD4. Interacts with EXO1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2; Turcot syndrome, an autosomal dominant disorder characterized by malignant tumors of the brain; Muir-Torre syndrome (MTS), a rare autosomal dominant disorder characterized by sebaceous neoplasms and visceral malignancy; lobular carcinoma in situ; endometrial cancer; and non-polyposis colorectal cancer.

Protein type: DNA repair, damage; Tumor suppressor

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: male germ cell nucleus; nucleoplasm; MutLalpha complex; chiasma; membrane; synaptonemal complex; nucleus

Molecular Function: protein binding; ATPase activity; guanine/thymine mispair binding; MutSalpha complex binding; single-stranded DNA binding; ATP binding

Biological Process: oogenesis; mismatch repair; somatic hypermutation of immunoglobulin genes; negative regulation of mitotic recombination; isotype switching; spindle midzone assembly involved in meiosis; DNA repair; double-strand break repair via nonhomologous end joining; poly(A) tail shortening; meiotic metaphase I plate congression; DNA damage response, signal transduction resulting in induction of apoptosis; synapsis; male meiosis chromosome segregation; resolution of meiotic joint molecules as recombinants; spermatogenesis

Disease: Muir-torre Syndrome; Mismatch Repair Cancer Syndrome; Colorectal Cancer, Hereditary Nonpolyposis, Type 2