Human Mucin3 ELISA Kit | MUC3 elisa kit

Human Mucin3 (MUC3) ELISA Kit

Cat. Num. AAA284969

• Gene Names: MUC17; MUC3; MUC-3; MUC-17
• Reactivity: Human
• Synonyms: Mucin3; Human Mucin3 (MUC3) ELISA Kit; MUC3; membrane mucin MUC17|mucin 17|secreted mucin MUC17|small intestinal mucin MUC3; MUC3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human MUC17. No significant cross-reactivity or interference between Human MUC17 and analogues was observed.

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for MUC3 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MUC17 in samples. An antibody specific for MUC17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUC17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC17 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 91982772
• NCBI GeneID: 140453
• NCBI Accession #: NP_001035194.1
• NCBI GenBank Nucleotide #: NM_001040105.1
• UniProt Accession #: Q685J3; O14761; Q685J2; Q8TDH7
• Molecular Weight: 436,777 Da
• NCBI Official Full Name: mucin-17
• NCBI Official Synonym Full Names: mucin 17, cell surface associated
• NCBI Official Symbol: MUC17
• NCBI Official Synonym Symbols: MUC3; MUC-3; MUC-17
• NCBI Protein Information: mucin-17
• UniProt Protein Name: Mucin-17
• UniProt Gene Name: MUC17
• UniProt Synonym Gene Names: MUC3; MUC-17; MUC-3

NCBI Description

The protein encoded by this gene is a membrane-bound mucin that provides protection to gut epithelial cells. The encoded protein contains about 60 tandem repeats, with each repeat being around 60 aa. N-glycosylation enables the encoded protein to localize on the cell surface, while the C-terminus interacts with the scaffold protein PDZ domain containing 1 (PDZK1). Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

MUC17: Probably plays a role in maintaining homeostasis on mucosal surfaces. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: apical plasma membrane; external side of plasma membrane; extracellular region; Golgi lumen; integral component of membrane; plasma membrane

Molecular Function: extracellular matrix constituent, lubricant activity; PDZ domain binding; protein binding

Biological Process: cellular homeostasis; O-glycan processing; stimulatory C-type lectin receptor signaling pathway