Rabbit RECK Polyclonal Antibody | anti-RECK antibody

RECK Polyclonal Antibody

Cat. Num. AAA2555913

• Gene Names: RECK; ST15
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: RECK; Polyclonal Antibody; RECK Polyclonal Antibody; hRECK; Membrane anchored glycoprotein (metastasis and invasion); RECK protein; Reversion inducing cysteine rich protein with Kazal motifs; Reversion-inducing cysteine-rich protein with Kazal motifs; ST15; Suppression of tumorigenicity 15 (reversion inducing cysteine rich protein with kazal motifs); Suppressor of tumorigenicity 15; Suppressor of tumorigenicity 15 protein; anti-RECK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 971

• Applicable Applications for anti-RECK antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant protein of human RECK
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using RECK antibody.)

Related Product Information for anti-RECK antibody

The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. Several transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 11863156
• NCBI GeneID: 8434
• NCBI Accession #: NP_066934.1
• NCBI GenBank Nucleotide #: NM_021111.3
• UniProt Accession #: O95980; Q5W0K6; Q8WX37; B2RNS1
• Molecular Weight: Calculated: 106kDa; Observed: 110kDa
• NCBI Official Full Name: reversion-inducing cysteine-rich protein with Kazal motifs isoform 1
• NCBI Official Synonym Full Names: reversion inducing cysteine rich protein with kazal motifs
• NCBI Official Symbol: RECK
• NCBI Official Synonym Symbols: ST15
• NCBI Protein Information: reversion-inducing cysteine-rich protein with Kazal motifs
• UniProt Protein Name: Reversion-inducing cysteine-rich protein with Kazal motifs
• Protein Family: Reversion-inducing cysteine-rich protein
• UniProt Gene Name: RECK
• UniProt Synonym Gene Names: ST15ImportedManual assertion inferred from database entriesiEMBL:BAA34060.1; hRECK1 PublicationManual assertion based on opinion iniRef.1

NCBI Description

The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Uniprot Description

Functions together with ADGRA2 to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed:28289266, PubMed:30026314). Plays a key role in Wnt7-specific responses: required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (). Acts as a Wnt7-specific coactivator of canonical Wnt signaling by decoding Wnt ligands: acts by interacting specifically with the disordered linker region of Wnt7, thereby conferring ligand selectivity for Wnt7 (PubMed:30026314). ADGRA2 is then required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (PubMed:30026314). Also acts as a serine protease inhibitor: negatively regulates matrix metalloproteinase-9 (MMP9) by suppressing MMP9 secretion and by direct inhibition of its enzymatic activity (PubMed:9789069, PubMed:18194466). Also inhibits metalloproteinase activity of MMP2 and MMP14 (MT1-MMP) (PubMed:9789069).