Human AF-10 ELISA Kit | MLLT10 elisa kit

Human Protein AF-10 (MLLT10) ELISA Kit

Cat. Num. AAA284779

• Gene Names: MLLT10; AF10
• Reactivity: Human
• Synonyms: AF-10; Human Protein AF-10 (MLLT10) ELISA Kit; RP11-399C16.2; AF10; DKFZp686E10210; MGC75086; ALL-1 fused gene from chromosome 10|myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to; 10|myeloid/lymphoid or mixed-linea; MLLT10 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human MLLT10. No significant cross-reactivity or interference between Human MLLT10 and analogues was observed.

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for MLLT10 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MLLT10 in samples. An antibody specific for MLLT10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMLLT10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MLLT10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MLLT10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 307548832
• NCBI GeneID: 8028
• NCBI Accession #: NP_001182555.1
• NCBI GenBank Nucleotide #: NM_001195626.1
• UniProt Accession #: P55197; Q5JT37; Q5VX90; Q66K63; B1ANA8
• Molecular Weight: 20,047 Da
• NCBI Official Full Name: protein AF-10 isoform c
• NCBI Official Synonym Full Names: MLLT10, histone lysine methyltransferase DOT1L cofactor
• NCBI Official Symbol: MLLT10
• NCBI Official Synonym Symbols: AF10
• NCBI Protein Information: protein AF-10
• UniProt Protein Name: Protein AF-10
• Protein Family: Protein
• UniProt Gene Name: MLLT10
• UniProt Synonym Gene Names: AF10

NCBI Description

This gene encodes a transcription factor and has been identified as a partner gene involved in several chromosomal rearrangements resulting in various leukemias. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]

Uniprot Description

MLLT10: Probably involved in transcriptional regulation. In vitro or as fusion protein with MLL has transactivation activity. Binds to cruciform DNA. A chromosomal aberration involving MLLT10 is associated with acute leukemias. Translocation t(10;11)(p12;q23) with MLL/HRX. The result is a rogue activator protein. A chromosomal aberration involving MLLT10 is associated with diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with PICALM. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 10p12.31

Cellular Component: cytosol; nucleoplasm; nucleus; protein complex

Molecular Function: DNA binding; DNA binding transcription factor activity; metal ion binding; protein binding

Biological Process: positive regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent

Disease: Leukemia, Acute Myeloid