Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein Plunc (PLUNC) Standard Curve.)
Mouse Protein Plunc (PLUNC) ELISA Kit | PLUNC elisa kit
Mouse Protein Plunc (PLUNC) ELISA Kit
Gene Names
Bpifa1; LUNX; NASG; Plunc; SPURT; SPLUNC1
Reactivity
Mouse
Synonyms
Protein Plunc (PLUNC); Mouse Protein Plunc (PLUNC) ELISA Kit; PLUNC; LPLUNC3; LUNX; NASG; SPLUNC1; SPURT; bA49G10.5; OTTHUMP00000030627; ligand-binding protein RYA3; nasopharyngeal carcinoma-related protein; secretory protein in upper respiratory tracts; tracheal epithel; PLUNC elisa kit
Reactivity
Mouse
Specificity
Mouse Protein Plunc (PLUNC) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLUNC. No significant cross-reactivity or interference between Mouse PLUNC and analogues was observed.
Sequence Length
278
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein Plunc (PLUNC) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein Plunc (PLUNC) Standard Curve.)
Related Product Information for PLUNC elisa kit
Description: This Mouse Protein Plunc (PLUNC) ELISA Kit employs a two-site sandwich ELISA to quantitate PLUNC in samples. An antibody specific for PLUNC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLUNC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLUNC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLUNC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PLUNC is the human homolog of murine plunc, and like the mouse gene, is specifically expressed in the upper airways and nasopharyngeal regions. The exact biological function of this gene is not known, however, it has been suggested to be involved in inflammatory responses to irritants in the upper airways. It may also serve as a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer. Multiple transcript variants resulting from alternative splicing in the 3' UTR have been detected, but the full-length nature of only two is known.The cDNA encodes a leucine-rich protein of 256 amino acids that is 72% identical to the murine protein. within the human PLUNC family, amino acid sequence identity is low, ranging from 16 to 28%.
Background: PLUNC is the human homolog of murine plunc, and like the mouse gene, is specifically expressed in the upper airways and nasopharyngeal regions. The exact biological function of this gene is not known, however, it has been suggested to be involved in inflammatory responses to irritants in the upper airways. It may also serve as a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer. Multiple transcript variants resulting from alternative splicing in the 3' UTR have been detected, but the full-length nature of only two is known.The cDNA encodes a leucine-rich protein of 256 amino acids that is 72% identical to the murine protein. within the human PLUNC family, amino acid sequence identity is low, ranging from 16 to 28%.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
BPI fold-containing family A member 1
NCBI Official Synonym Full Names
BPI fold containing family A, member 1
NCBI Official Symbol
Bpifa1
NCBI Official Synonym Symbols
LUNX; NASG; Plunc; SPURT; SPLUNC1
NCBI Protein Information
BPI fold-containing family A member 1
UniProt Protein Name
BPI fold-containing family A member 1
UniProt Gene Name
Bpifa1
UniProt Synonym Gene Names
Plunc; Splunc1
Uniprot Description
Plays a role in the innate immune responses of the upper airways. Reduces the surface tension in secretions from airway epithelia and inhibits the formation of biofilm by pathogenic Gram-negative bacteria, such as P.aeruginosa and K.pneumoniae. Binds bacterial lipopolysaccharide (LPS). Negatively regulates proteolytic cleavage of SCNN1G, an event that is required for activation of the epithelial sodium channel (ENaC), and thereby contributes to airway surface liquid homeostasis and proper clearance of mucus (). Plays a role in the airway inflammatory response after exposure to irritants. May attract macrophages and neutrophils. May be associated with tumor progression.
Research Articles on PLUNC
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Product Notes
The Mouse PLUNC bpifa1 (Catalog #AAA9717332) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717332 ELISA Kit recognizes Mouse PLUNC. It is sometimes possible for the material contained within the vial of "Protein Plunc (PLUNC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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