Rat Adenylyl cyclase-associated protein 1 ELISA Kit | CAP1 elisa kit

Rat Adenylyl cyclase-associated protein 1 (CAP1) ELISA Kit

Cat. Num. AAA284368

• Gene Names: Cap1; Mch1
• Reactivity: Rat
• Synonyms: Adenylyl cyclase-associated protein 1; Rat Adenylyl cyclase-associated protein 1 (CAP1) ELISA Kit; RP11-115D7.1; CAP; CAP1-PEN; OTTHUMP00000004822|adenylyl cyclase-associated protein; CAP1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat CAP1. No significant cross-reactivity or interference between Rat CAP1 and analogues was observed.
• Sequence Length: 475

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for CAP1 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CAP1 in samples. An antibody specific for CAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 59709467
• NCBI GeneID: 64185
• NCBI Accession #: NP_071778.2
• NCBI GenBank Nucleotide #: NM_022383.2
• UniProt Accession #: Q08163; Q5FVS8
• Molecular Weight: 51,589 Da
• NCBI Official Full Name: adenylyl cyclase-associated protein 1
• NCBI Official Synonym Full Names: cyclase associated actin cytoskeleton regulatory protein 1
• NCBI Official Symbol: Cap1
• NCBI Official Synonym Symbols: Mch1
• NCBI Protein Information: adenylyl cyclase-associated protein 1
• UniProt Protein Name: Adenylyl cyclase-associated protein 1
• Protein Family: AP-1-like transcription factor
• UniProt Gene Name: Cap1
• UniProt Synonym Gene Names: Cap; Mch1; CAP 1

NCBI Description

associated with the S. cerevisia adenylyl cyclase-assoiciated protein (CAP); may be involved in the cyclic AMP signalling pathway [RGD, Feb 2006]

Uniprot Description

CAP1: an actin binding protein. Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.

Protein type: Actin-binding; Cytoskeletal

Chromosomal Location of Human Ortholog: 5q36

Cellular Component: cortical actin cytoskeleton; cytoplasm; extracellular space; focal adhesion; plasma membrane

Molecular Function: actin binding; adenylate cyclase binding

Biological Process: actin cytoskeleton organization; actin polymerization or depolymerization; ameboidal cell migration; cell morphogenesis; establishment and/or maintenance of cell polarity; receptor-mediated endocytosis; regulation of adenylate cyclase activity; signal transduction