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Immunohistochemistry (IHC) (Immunochemical staining of mouse ICAM1 in mouse kidney with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to blood vessel.)

Rabbit anti-Mouse ICAM-1 Monoclonal Antibody | anti-ICAM1 antibody

Recombinant Anti-ICAM-1 Antibody, Rabbit Monoclonal

Gene Names
Icam1; CD54; Ly-47; Icam-1; MALA-2
Reactivity
Mouse
Applications
ELISA, ELISA, Immunohistochemistry
Purity
Protein A
Synonyms
ICAM-1; Monoclonal Antibody; Recombinant Anti-ICAM-1 Antibody; Rabbit Monoclonal; ICAM-1/CD54 Antibody; Rabbit MAb; intercellular adhesion molecule 1; Anti-CD54 Antibody; Anti-Icam-1 Antibody; Anti-Ly-47 Antibody; Anti-MALA-2 Antibody; anti-ICAM1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
020
Specificity
Mouse CD54/ICAM1.
No cross-reactivity in ELISA with Human ICAM1, Human CD31, Human ICAM3, Human ICAM2, Mouse VCAM1
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ICAM1 antibody
ELISA (EIA), ELISA (EIA) (Cap), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
ELISA: 1:5000-1:10,000
IHC-P: 1:100-1:500
ELISA (Cap): 1:250-1:2000
ELISA (Cap): This antibody will detect Mouse ICAM-1/CD54 in ELISA pair set.
In a sandwich ELISA, it can be used as capture antibody when paired with.
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse CD54/ICAM1 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD54/ICAM1 (rM CD54/ICAM1).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse ICAM1 in mouse kidney with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to blood vessel.)

Immunohistochemistry (IHC) (Immunochemical staining of mouse ICAM1 in mouse kidney with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to blood vessel.)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse ICAM1 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). The image showing positive staining of choroid plexus.)

Immunohistochemistry (IHC) (Immunochemical staining of mouse ICAM1 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). The image showing positive staining of choroid plexus.)
Related Product Information for anti-ICAM1 antibody
Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.
Product Categories/Family for anti-ICAM1 antibody
References
Xu H, et al. (2001) The role of ICAM-1 molecule in the migration of Langerhans cells in the skin and regional lymph node. Eur J Immunol. 31(10): 3085-93.Terol MJ, et al. (2003) Soluble intercellular adhesion molecule-1 (s-ICAM-1/s-CD54) in diffuse large B-cell lymphoma: association with clinical characteristics and outcome. Ann Oncol. 14(3): 467-74.Mendez MP, et al. (2006) Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury. Am J Physiol Lung Cell Mol Physiol. 290(5): L962-70.Lawson C, et al. (2009) ICAM-1 signaling in endothelial cells. Pharmacol Rep. 61(1): 22-32.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
58,753 Da
NCBI Official Full Name
intercellular adhesion molecule 1
NCBI Official Synonym Full Names
intercellular adhesion molecule 1
NCBI Official Symbol
Icam1
NCBI Official Synonym Symbols
CD54; Ly-47; Icam-1; MALA-2
NCBI Protein Information
intercellular adhesion molecule 1; myD10
UniProt Protein Name
Intercellular adhesion molecule 1
UniProt Gene Name
Icam1
UniProt Synonym Gene Names
Icam-1; ICAM-1
UniProt Entry Name
ICAM1_MOUSE

Uniprot Description

ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta.

Protein type: Cell adhesion; Membrane protein, integral; Immunoglobulin superfamily

Cellular Component: extracellular space; cell surface; focal adhesion; membrane; integral to plasma membrane; integral to membrane; plasma membrane; immunological synapse; external side of plasma membrane; lipid raft

Molecular Function: integrin binding; protein complex binding

Biological Process: regulation of cell adhesion; virion attachment, binding of host cell surface receptor; negative regulation of calcium ion transport; positive regulation of nitric oxide biosynthetic process; T cell antigen processing and presentation; activation of NF-kappaB transcription factor; positive regulation of cellular extravasation; regulation of cell shape; cell-cell adhesion; leukocyte adhesion; positive regulation of peptidyl-tyrosine phosphorylation; sensory perception of sound; positive regulation of actin filament polymerization; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; positive regulation of vasoconstriction; adhesion to host; cell adhesion mediated by integrin; cell adhesion; acute inflammatory response to antigenic stimulus

Research Articles on ICAM1

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Product Notes

The ICAM1 icam1 (Catalog #AAA8111570) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-ICAM-1 Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ICAM-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), ELISA (EIA) (Cap), Immunohistochemistry-Paraffin (IHC-P). ELISA: 1:5000-1:10,000 IHC-P: 1:100-1:500 ELISA (Cap): 1:250-1:2000 ELISA (Cap): This antibody will detect Mouse ICAM-1/CD54 in ELISA pair set. In a sandwich ELISA, it can be used as capture antibody when paired with. Researchers should empirically determine the suitability of the ICAM1 icam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ICAM-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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