Rabbit anti-Human ALOX5AP Polyclonal Antibody | anti-ALOX5AP antibody

ALOX5AP, ID (Arachidonate 5-lipoxygenase-activating protein, FLAP, MK-886-binding Protein) (MaxLight 550)

Cat. Num. AAA6462519

• Gene Names: ALOX5AP; FLAP
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ALOX5AP; Polyclonal Antibody; ID (Arachidonate 5-lipoxygenase-activating protein; FLAP; MK-886-binding Protein) (MaxLight 550); anti-ALOX5AP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ALOX5AP.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
• Sequence Length: 161

• Applicable Applications for anti-ALOX5AP antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human ALOX5AP (KLH).
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-ALOX5AP Antibody (Center) in K562 cell line lysates (35ug/lane). ALOX5AP(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALOX5AP antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-ALOX5AP antibody

Antibodies; Leukotrienes

References

1. Zintzaras, E., Am. J. Epidemiol. 169(5), 523-532 (2009). 2. Crosslin, D.R., Hum. Genet. 125(2), 217-229 (2009). 3. Steinhilber, D., Pharm Acta Helv 69(1), 3-14 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 15718675
• NCBI GeneID: 241
• NCBI Accession #: NP_001620
• NCBI GenBank Nucleotide #: NP_001620.2
• UniProt Accession #: P20292
• Molecular Weight: 18.157
• NCBI Official Full Name: arachidonate 5-lipoxygenase-activating protein isoform 1
• NCBI Official Synonym Full Names: arachidonate 5-lipoxygenase activating protein
• NCBI Official Symbol: ALOX5AP
• NCBI Official Synonym Symbols: FLAP
• NCBI Protein Information: arachidonate 5-lipoxygenase-activating protein
• UniProt Protein Name: Arachidonate 5-lipoxygenase-activating protein
• Protein Family: Arachidonate 5-lipoxygenase-activating protein
• UniProt Gene Name: ALOX5AP
• UniProt Synonym Gene Names: FLAP
• UniProt Entry Name: AL5AP_HUMAN

NCBI Description

This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]