Insulin-like Growth Factor 1 ELISA Kit | IGF-1 elisa kit

Mouse Insulin-like Growth Factor 1 (IGF-1) ELISA Kit

Cat. Num. AAA282031

• Gene Names: Furin; Fur; PACE; SPC1; Pcsk3; 9130404I01Rik
• Synonyms: Insulin-like Growth Factor 1; Mouse Insulin-like Growth Factor 1 (IGF-1) ELISA Kit; IGF-1 elisa kit

• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse FURIN. No significant cross-reactivity or interference between Mouse FURIN and analogues was observed.
• Sequence Length: 154

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for IGF-1 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate FURIN in samples. An antibody specific for FURIN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFURIN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FURIN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FURIN bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 125347022
• NCBI GeneID: 18550
• NCBI Accession #: NP_001074923.1
• NCBI GenBank Nucleotide #: NM_001081454.2
• UniProt Accession #: P23188; Q6GTN6
• Molecular Weight: 86,772 Da
• NCBI Official Full Name: furin preproprotein
• NCBI Official Synonym Full Names: furin (paired basic amino acid cleaving enzyme)
• NCBI Official Symbol: Furin
• NCBI Official Synonym Symbols: Fur; PACE; SPC1; Pcsk3; 9130404I01Rik
• NCBI Protein Information: furin
• UniProt Protein Name: Furin
• Protein Family: Insulin-like growth factor 1 receptor
• UniProt Gene Name: Furin
• UniProt Synonym Gene Names: Fur; Pcsk3; PACE

NCBI Description

This gene encodes a calcium-dependent serine endoprotease that proteolytically activates different proprotein substrates traversing the secretory pathway. The encoded protein undergoes proteolytic autoactivation during which an N-terminal propeptide is cleaved to generate the mature protein. Mice lacking the encoded protein die at an embryonic stage and display hemodynamic insufficiency, cardiac ventral closure defect, axial rotation defect and abnormal yolk sac vasculature. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

FURIN: Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Belongs to the peptidase S8 family. Furin subfamily.

Protein type: Cell surface; EC 3.4.21.75; Membrane protein, integral; Protease; Vesicle

Chromosomal Location of Human Ortholog: 7 D2|7 45.65 cM

Cellular Component: cell surface; early endosome; endoplasmic reticulum; endoplasmic reticulum membrane; endosome; extracellular region; Golgi apparatus; Golgi cisterna; Golgi membrane; integral component of membrane; membrane; membrane raft; plasma membrane; trans-Golgi network; trans-Golgi network transport vesicle; trans-Golgi network transport vesicle membrane

Molecular Function: endopeptidase activity; hydrolase activity; metal ion binding; nerve growth factor binding; peptidase activity; peptide binding; protease binding; protein binding; serine-type endopeptidase activity; serine-type endopeptidase inhibitor activity; serine-type peptidase activity

Biological Process: cell proliferation; negative regulation of low-density lipoprotein particle receptor catabolic process; negative regulation of transforming growth factor beta1 production; nerve growth factor production; peptide biosynthetic process; peptide hormone processing; positive regulation of cell migration; positive regulation of transforming growth factor beta receptor signaling pathway; protein processing; proteolysis; regulation of endopeptidase activity; regulation of low-density lipoprotein particle receptor biosynthetic process; regulation of protein catabolic process; regulation of signal transduction; secretion by cell; signal peptide processing; zymogen activation