Mouse FUR (Furin) ELISA Kit | FUR elisa kit

Mouse FUR (Furin) ELISA Kit

Cat. Num. AAA7606595

• Gene Names: Furin; Fur; PACE; SPC1; Pcsk3; 9130404I01Rik
• Reactivity: Mouse
• Synonyms: FUR (Furin); Mouse FUR (Furin) ELISA Kit; FUR elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of FUR . No significant cross-reactivity or interference between FUR and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between FUR and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 793

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Detection Range: 46.88-3000pg/ml
• Sensitivity: <28.125pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FUR were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FUR were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FUR elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- FUR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- FUR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FUR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FUR can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 341940726
• NCBI GeneID: 18550
• UniProt Accession #: P23188; Q6GTN6
• Molecular Weight: 86,772 Da
• NCBI Official Full Name: Furin
• NCBI Official Synonym Full Names: furin (paired basic amino acid cleaving enzyme)
• NCBI Official Symbol: Furin
• NCBI Official Synonym Symbols: Fur; PACE; SPC1; Pcsk3; 9130404I01Rik
• NCBI Protein Information: furin
• UniProt Protein Name: Furin
• Protein Family: Ferric uptake regulation protein
• UniProt Gene Name: Furin
• UniProt Synonym Gene Names: Fur; Pcsk3; PACE

NCBI Description

This gene encodes a calcium-dependent serine endoprotease that proteolytically activates different proprotein substrates traversing the secretory pathway. The encoded protein undergoes proteolytic autoactivation during which an N-terminal propeptide is cleaved to generate the mature protein. Mice lacking the encoded protein die at an embryonic stage and display hemodynamic insufficiency, cardiac ventral closure defect, axial rotation defect and abnormal yolk sac vasculature. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

FURIN: Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Belongs to the peptidase S8 family. Furin subfamily.

Protein type: Cell surface; EC 3.4.21.75; Membrane protein, integral; Protease; Vesicle

Chromosomal Location of Human Ortholog: 7 D2|7 45.65 cM

Cellular Component: cell surface; early endosome; endoplasmic reticulum; endoplasmic reticulum membrane; Golgi cisterna; Golgi membrane; integral to membrane; lipid raft; membrane; plasma membrane; trans-Golgi network; trans-Golgi network transport vesicle; trans-Golgi network transport vesicle membrane

Molecular Function: endopeptidase activity; nerve growth factor binding; peptidase activity; peptide binding; protease binding; protein binding; serine-type endopeptidase activity; serine-type endopeptidase inhibitor activity

Biological Process: cell proliferation; negative regulation of low-density lipoprotein receptor catabolic process; negative regulation of transforming growth factor-beta1 production; nerve growth factor production; peptide biosynthetic process; peptide hormone processing; positive regulation of cell migration; positive regulation of transforming growth factor beta receptor signaling pathway; protein processing; regulation of endopeptidase activity; regulation of low-density lipoprotein receptor biosynthetic process; regulation of protein catabolic process; regulation of signal transduction; secretion by cell; signal peptide processing; zymogen activation