Human Potassium voltage-gated channel subfamily E member 2 ELISA Kit | KCNE2 elisa kit

Human Potassium voltage-gated channel subfamily E member 2 (KCNE2) ELISA Kit

Cat. Num. AAA281159

• Gene Names: KCNE2; LQT5; LQT6; ATFB4; MIRP1
• Reactivity: Human
• Synonyms: Potassium voltage-gated channel subfamily E member 2; Human Potassium voltage-gated channel subfamily E member 2 (KCNE2) ELISA Kit; KCNE2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human KCNE2. No significant cross-reactivity or interference between Human KCNE2 and analogues was observed.
• Sequence Length: 123

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for KCNE2 elisa kit

Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume.
KCNE2 encodes a member of the potassium channel, voltage-gated, isk-related subfamily. This member is a small integral membrane subunit that assembles with the KCNH2 gene product, a pore-forming protein, to alter its function. This gene is expressed in heart and muscle and the gene mutations are associated with cardiac arrhythmia.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate KCNE2 in samples. An antibody specific for KCNE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKCNE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KCNE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KCNE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 27436978
• NCBI GeneID: 9992
• NCBI Accession #: NP_751951.1
• NCBI GenBank Nucleotide #: NM_172201.1
• UniProt Accession #: Q9Y6J6; Q52LJ5; A5H1P3; D3DSF8
• Molecular Weight: 14,472 Da
• NCBI Official Full Name: potassium voltage-gated channel subfamily E member 2
• NCBI Official Synonym Full Names: potassium voltage-gated channel subfamily E regulatory subunit 2
• NCBI Official Symbol: KCNE2
• NCBI Official Synonym Symbols: LQT5; LQT6; ATFB4; MIRP1
• NCBI Protein Information: potassium voltage-gated channel subfamily E member 2
• UniProt Protein Name: Potassium voltage-gated channel subfamily E member 2
• Protein Family: Potassium voltage-gated channel subfamily
• UniProt Gene Name: KCNE2

NCBI Description

Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, isk-related subfamily. This member is a small integral membrane subunit that assembles with the KCNH2 gene product, a pore-forming protein, to alter its function. This gene is expressed in heart and muscle and the gene mutations are associated with cardiac arrhythmia. [provided by RefSeq, Jul 2008]

Uniprot Description

KCNE2: Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Associated with KCNH2/HERG is proposed to form the rapidly activating component of the delayed rectifying potassium current in heart (IKr). May associate with KCNQ2 and/or KCNQ3 and modulate the native M-type current. May associate with KCNQ1/KVLTQ1 and elicit a voltage-independent current. May associate with HCN1 and HCN2 and increase potassium current. Defects in KCNE2 are the cause of long QT syndrome type 6 (LQT6). Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. KCNE2 mutants form channels that open slowly and close rapidly, thereby diminishing potassium currents. Defects in KCNE2 are the cause of familial atrial fibrillation type 4 (ATFB4). Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the potassium channel KCNE family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: cell surface; endoplasmic reticulum; Golgi apparatus; lysosome; plasma membrane; voltage-gated potassium channel complex

Molecular Function: delayed rectifier potassium channel activity; inward rectifier potassium channel activity; potassium channel regulator activity; protein binding; protein homodimerization activity

Biological Process: aging; potassium ion import; tongue development

Disease: Atrial Fibrillation, Familial, 4; Long Qt Syndrome 6