Mouse Vimentin (VIM) ELISA Kit | VIM elisa kit

Mouse Vimentin (VIM) ELISA Kit

Cat. Num. AAA9716632

• Reactivity: Mouse
• Synonyms: Vimentin (VIM); Mouse Vimentin (VIM) ELISA Kit; VIM; RP11-124N14.1; FLJ36605; VIM elisa kit

• Reactivity: Mouse
• Specificity: Mouse Vimentin (VIM) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse VIM. No significant cross-reactivity or interference between Mouse VIM and analogues was observed.
• Sequence Length: 466

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Vimentin (VIM) Standard Curve.)

Related Product Information for VIM elisa kit

Description: This Mouse Vimentin (VIM) ELISA Kit employs a two-site sandwich ELISA to quantitate VIM in samples. An antibody specific for VIM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VIM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VIM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VIM bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprise the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression. A vimentin monomer, like all other intermediate filaments, has a central alpha-helical domain, capped on each end by non-helical amino (head) and carboxyl (tail) domains.

NCBI and Uniprot Product Information

• NCBI GI #: 31982755
• NCBI GeneID: 22352
• NCBI Accession #: NP_035831.2
• NCBI GenBank Nucleotide #: NP_035831.2
• UniProt Accession #: P20152
• NCBI Official Full Name: vimentin
• NCBI Official Synonym Full Names: vimentin
• NCBI Official Symbol: Vim
• NCBI Protein Information: vimentin
• UniProt Protein Name: Vimentin
• Protein Family: Vimentin
• UniProt Gene Name: Vim

Uniprot Description

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.