Human Reticulocalbin-3 (RCN3) ELISA Kit | RCN3 elisa kit

Human Reticulocalbin-3 (RCN3) ELISA Kit

Cat. Num. AAA2802070

• Gene Names: RCN3; RLP49
• Reactivity: Human
• Synonyms: Reticulocalbin-3 (RCN3); Human Reticulocalbin-3 (RCN3) ELISA Kit; RLP49; reticulocabin|reticulocalbin-like; RCN3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human RCN3. No significant cross-reactivity or interference between Human RCN3 and analogues was observed.

• Assay Type: Sandwich
• Assay: Serum, Plasma, Other biological fluids
• Analysis Method: Quantitive
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Assay Duration: 1-4.5h
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate RCN3 in samples. An antibody specific for RCN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRCN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RCN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RCN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Preparation and Storage: Store at 2-8 degree C. Please refer to Instruction Manual.

Related Product Information for RCN3 elisa kit

Sequence analysis indicates that the 48kDa protein is a putative Ca2+-binding protein, RCN-3 (reticulocalbin-3), which had previously been predicted by bioinformatic analysis of cDNA from the human hypothalamus. RCN-3 is a member of the CREC family of multiple EF-hand Ca2+-binding proteins localized to the secretory pathway.
The most interesting feature of the RCN-3 sequence is the presence of five Arg-Xaa-Xaa-Arg motifs, which represents the target sequence of SPCs. Biosynthetic studies showed that RCN-3 is transiently associated with proPACE4, but not with mature PACE4. Inhibition of PACE4 maturation by a Ca2+ ionophore resulted in accumulation of the proPACE4-RCN-3 complex in cells. Furthermore, autoactivation and secretion of PACE4 was increased upon co-expression with RCN-3.

NCBI and Uniprot Product Information

• NCBI GI #: 28626510
• NCBI GeneID: 57333
• NCBI Accession #: NP_065701.2
• NCBI GenBank Nucleotide #: NM_020650.2
• UniProt Accession #: Q96D15; Q9HBZ8
• Molecular Weight: 37,493 Da
• NCBI Official Full Name: reticulocalbin-3
• NCBI Official Synonym Full Names: reticulocalbin 3, EF-hand calcium binding domain
• NCBI Official Symbol: RCN3
• NCBI Official Synonym Symbols: RLP49
• NCBI Protein Information: reticulocalbin-3; EF-hand calcium-binding protein RLP49; reticulocabin
• UniProt Protein Name: Reticulocalbin-3
• Protein Family: Reticulocalbin
• UniProt Gene Name: RCN3
• UniProt Entry Name: RCN3_HUMAN

Uniprot Description

RCN3: Belongs to the CREC family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: endoplasmic reticulum lumen

Molecular Function: protein binding; calcium ion binding