Reticulocalbin 3/RCN3 Recombinant Protein | RCN3 recombinant protein

Reticulocalbin 3/RCN3 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121450

• Gene Names: RCN3; RLP49
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Reticulocalbin 3/RCN3; Reticulocalbin 3/RCN3 Protein; Human; Recombinant (His Tag); Human RCN3 Protein (His Tag); RLP49 Protein; reticulocalbin 3; EF-hand calcium binding domain; RCN3 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His324

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Lys 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human RCN3 (Q96D15)(Met1-His324) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for RCN3 recombinant protein

Background: RCN3 belongs to the CREC family which contains multiple EF-hand Ca2+-binding proteins localized to the secretory pathway. RCN3 sequence is characterized by the presence of five Arg-Xaa-Xaa-Arg motifs, which represents the target sequence of subtilisin-like proprotein convertases(SPCs). SPCs are a family of seven structurally related serine endoproteases that are involved in the proteolytic activation of proproteins.RCN3 is transiently associated with proPACE4, but not with mature PACE4. Inhibition of PACE4 maturation by a Ca2+ ionophore resulted in accumulation of the proPACE4-RCN-3 complex in cells. It has been proposed that elective and transient association of RCN3 with the precursor of PACE4 plays an important role in the biosynthesis of PACE4.

References

Danielsen JM, et al. (2011) Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level. Mol Cell Proteomics. 10(3):M110.003590. Rual JF, et al. (2005) Towards a proteome-scale map of the human protein-protein interaction network. Nature. 437(7062):1173-8. Kamatani Y, et al. (2010) Genome-wide association study of hematological and biochemical traits in a Japanese population. Nat Genet. 42(3):210-5.

NCBI and Uniprot Product Information

• NCBI GI #: 28626510
• NCBI GeneID: 57333
• NCBI Accession #: NP_065701.2
• NCBI GenBank Nucleotide #: NM_020650.2
• UniProt Accession #: Q96D15; Q9HBZ8
• Molecular Weight: 37,493 Da
• NCBI Official Full Name: reticulocalbin-3
• NCBI Official Synonym Full Names: reticulocalbin 3, EF-hand calcium binding domain
• NCBI Official Symbol: RCN3
• NCBI Official Synonym Symbols: RLP49
• NCBI Protein Information: reticulocalbin-3; EF-hand calcium-binding protein RLP49; reticulocabin
• UniProt Protein Name: Reticulocalbin-3
• Protein Family: Reticulocalbin
• UniProt Gene Name: RCN3
• UniProt Entry Name: RCN3_HUMAN

Uniprot Description

RCN3: Belongs to the CREC family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: endoplasmic reticulum lumen

Molecular Function: protein binding; calcium ion binding