Activity
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UBE2K (UBC1) Recombinant Protein | UBE2K recombinant protein
UBE2K (UBC1), Active
Applications
Western Blot
Synonyms
UBE2K (UBC1); Active; E2-25K; HIP2; HYPG; LIG; UBC1; UBE2K recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 50mM sodium phosphate, pH7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Applicable Applications for UBE2K recombinant protein
Ubiquitin Enzyme Assay, Western Blot (WB)
Tag
HIS
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for UBE2K recombinant protein
Recombinant full-length human UBE2K was expressed in E Coli cells using an N-terminal His tag. The UBE2K gene accession number is NM_005339.
UBE2K also known as UBC1 or ubiquitin-conjugating enzyme E2K belongs to the ubiquitin-conjugating enzyme family which interacts with RING finger proteins. UBE2K can ubiquitinate huntingtin, the gene product for Huntington's disease. Huntingtin is ubiquitinated in peripheral cells, suggesting a role for UBE2K in the degradation of huntingtin. UBE2K plays a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases. UBE2K also plays a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation.
UBE2K also known as UBC1 or ubiquitin-conjugating enzyme E2K belongs to the ubiquitin-conjugating enzyme family which interacts with RING finger proteins. UBE2K can ubiquitinate huntingtin, the gene product for Huntington's disease. Huntingtin is ubiquitinated in peripheral cells, suggesting a role for UBE2K in the degradation of huntingtin. UBE2K plays a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases. UBE2K also plays a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation.
Product Categories/Family for UBE2K recombinant protein
References
1. Kalchman, M.A. et al: Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme. J. Biol. Chem. 271: 19385-19394, 1996
2. De Pril, R. et al: Ubiquitin-conjugating enzyme E2-25K increases aggregate formation and cell death in polyglutamine diseases. Mol Cell Neurosci. 2007 Jan;34(1):10-9
2. De Pril, R. et al: Ubiquitin-conjugating enzyme E2-25K increases aggregate formation and cell death in polyglutamine diseases. Mol Cell Neurosci. 2007 Jan;34(1):10-9
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26kDa
Protein Family
Similar Products
Product Notes
The UBE2K (Catalog #AAA517752) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's UBE2K (UBC1) can be used in a range of immunoassay formats including, but not limited to, Ubiquitin Enzyme Assay, Western Blot (WB). Researchers should empirically determine the suitability of the UBE2K for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2K (UBC1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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