Mouse Myosin-4 (MYH4) ELISA Kit | MYH4 elisa kit

Mouse Myosin-4 (MYH4) ELISA Kit

Cat. Num. AAA2801359

• Gene Names: Myh4; MM; MHC2B; Myhsf; MYH-2B; Minmus; Minimsc; AI506973; MyHC-IIb
• Reactivity: Mouse
• Synonyms: Myosin-4 (MYH4); Mouse Myosin-4 (MYH4) ELISA Kit; MYH2B; MyHC-2B; MyHC-IIb; myosin; heavy polypeptide 4; skeletal muscle; MYH4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse MYH4. No significant cross-reactivity or interference between Mouse MYH4 and analogues was observed.
• Sequence Length: 1939

• Assay Type: Detection Method: Sandwich; Analysis Method: Quantitive
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Range: 15.6-1000 pg/mL
• Sensitivity: 6.4 pg/mL
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for MYH4 elisa kit

Test Principle: This assay employs a two-site sandwich ELISA to quantitate MYH4 in samples. An antibody specific for MYH4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYH4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYH4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYH4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Overview: All MHC proteins share approximately 100% conservation in the phosphate-binding loop, several helices forming the nucleotide-binding pocket, residues involved in actin binding, residues involved in the stereospecific hydrophobic rigor state interaction of actin with myosin, a cleft that divides the ATP- and actin-binding sites, and a converter domain, or fulcrum, containing 2 conserved cysteines.the MYH4 gene would appear to be located on 17p13.1, the location of 4 other myosin heavy chain genes (MYH1; MYH2; MYH3; and MYH8). Furthermore, Soussi-Yanicostas et al. (1993) had evidence from analysis of YACs that the embryonic and fetal genes, on the one hand, and the adult fast myosin heavy chain genes, on the other hand, are contained within different genomic fragments.

NCBI and Uniprot Product Information

• NCBI GI #: 73921192
• NCBI GeneID: 17884
• UniProt Accession #: Q5SX39; B9EJ73
• Molecular Weight: 222,859 Da
• NCBI Official Full Name: Myosin-4
• NCBI Official Synonym Full Names: myosin, heavy polypeptide 4, skeletal muscle
• NCBI Official Symbol: Myh4
• NCBI Official Synonym Symbols: MM; MHC2B; Myhsf; MYH-2B; Minmus; Minimsc; AI506973; MyHC-IIb
• NCBI Protein Information: myosin-4
• UniProt Protein Name: Myosin-4
• Protein Family: Myosin
• UniProt Gene Name: Myh4
• UniProt Synonym Gene Names: MyHC-2b

Uniprot Description

Muscle contraction.CautionRepresents a conventional myosin. This protein should not be confused with the unconventional myosin-4 (MYO4).