Human Smoothened (SMO) ELISA Kit | SMO elisa kit

Human Smoothened (SMO) ELISA Kit

Cat. Num. AAA2707601

• Gene Names: SMO; Gx; CRJS; SMOH; FZD11
• Reactivity: Human
• Synonyms: Smoothened (SMO); Human Smoothened (SMO) ELISA Kit; Gx; SMOH; SMO elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Smoothened (SMO).; No significant cross-reactivity or interference between Smoothened (SMO) and analogues was observed.
• Sequence Length: 787

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.059ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Smoothened (SMO) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Smoothened (SMO) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SMO elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Smoothened (SMO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Smoothened (SMO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Smoothened (SMO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Smoothened (SMO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5032099
• NCBI GeneID: 6608
• NCBI Accession #: NP_005622.1
• NCBI GenBank Nucleotide #: NP_005622.1
• UniProt Accession #: Q99835
• NCBI Official Full Name: smoothened homolog
• NCBI Official Synonym Full Names: smoothened, frizzled class receptor
• NCBI Official Symbol: SMO
• NCBI Official Synonym Symbols: Gx; CRJS; SMOH; FZD11
• NCBI Protein Information: smoothened homolog
• UniProt Protein Name: Smoothened homolog
• Protein Family: Smoothened
• UniProt Gene Name: SMO
• UniProt Synonym Gene Names: SMOH; SMO
• UniProt Entry Name: SMO_HUMAN

NCBI Description

The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. [provided by RefSeq, Jul 2010]

Uniprot Description

SMO: G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Membrane protein, multi-pass; Oncoprotein; GPCR, Fz/Smo family; Receptor, GPCR; Cell development/differentiation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q32.3

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; plasma membrane; integral to membrane; caveola

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; protein binding; Wnt receptor activity; patched binding; drug binding

Biological Process: developmental growth; central nervous system neuron differentiation; ventral midline determination; negative regulation of epithelial cell differentiation; skeletal muscle fiber development; cell fate specification; negative regulation of transcription from RNA polymerase II promoter; thalamus development; Wnt receptor signaling pathway through beta-catenin; anterior/posterior pattern formation; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; heart looping; vasculogenesis; activation of hh target transcription factor; neural crest cell migration; dorsoventral neural tube patterning; positive regulation of neuroblast proliferation; smoothened signaling pathway; hair follicle morphogenesis; protein stabilization; smoothened signaling pathway in regulation of granule cell precursor cell proliferation; in utero embryonic development; multicellular organism growth; smoothened signaling pathway in ventral spinal cord patterning; forebrain morphogenesis; negative regulation of hair follicle development; negative regulation of DNA binding; odontogenesis of dentine-containing teeth; osteoblast differentiation; G-protein coupled receptor protein signaling pathway; positive regulation of protein import into nucleus; cerebellar cortex morphogenesis; myoblast migration; midgut development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation; negative regulation of apoptosis