Human Protein Disulfide Isomerase A4 (PDIA4) ELISA Kit | PDIA4 elisa kit

Human Protein Disulfide Isomerase A4 (PDIA4) ELISA Kit

Cat. Num. AAA2705235

• Gene Names: PDIA4; ERP70; ERP72; ERp-72
• Reactivity: Human
• Synonyms: Protein Disulfide Isomerase A4 (PDIA4); Human Protein Disulfide Isomerase A4 (PDIA4) ELISA Kit; PDI-A4; ERP70; ERP72; Protein Disulfide Isomerase-Associated 4; Endoplasmic reticulum resident protein 70; PDIA4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase A4 (PDIA4).; No significant cross-reactivity or interference between Protein Disulfide Isomerase A4 (PDIA4) and analogues was observed.
• Sequence Length: 645

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.61ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Disulfide Isomerase A4 (PDIA4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Disulfide Isomerase A4 (PDIA4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDIA4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A4 (PDIA4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A4 (PDIA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase A4 (PDIA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase A4 (PDIA4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4758304
• NCBI GeneID: 9601
• NCBI Accession #: NP_004902.1
• NCBI GenBank Nucleotide #: NM_004911.4
• UniProt Accession #: P13667; Q549T6; A8K4K6
• Molecular Weight: 72,932 Da
• NCBI Official Full Name: protein disulfide-isomerase A4
• NCBI Official Synonym Full Names: protein disulfide isomerase family A member 4
• NCBI Official Symbol: PDIA4
• NCBI Official Synonym Symbols: ERP70; ERP72; ERp-72
• NCBI Protein Information: protein disulfide-isomerase A4
• UniProt Protein Name: Protein disulfide-isomerase A4
• Protein Family: Protein disulfide-isomerase
• UniProt Gene Name: PDIA4
• UniProt Synonym Gene Names: ERP70; ERP72; ER protein 70; ERp70; ER protein 72; ERp-72; ERp72

NCBI Description

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. This protein, when bound to cyclophilin B, enhances the rate of immunoglobulin G intermolecular disulfide bonding and antibody assembly. [provided by RefSeq, Dec 2016]