Mouse Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) ELISA Kit | CAMK2a elisa kit

Mouse Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) ELISA Kit

Cat. Num. AAA2705050

• Gene Names: CAMK2A; CAMKA; MRD53
• Reactivity: Mouse
• Synonyms: Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a); Mouse Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) ELISA Kit; CAMKA; CAMK2a elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a).; No significant cross-reactivity or interference between Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) and analogues was observed.
• Sequence Length: 478

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.112ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAMK2a elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 25952118
• NCBI GeneID: 815
• NCBI Accession #: NP_741960.1
• NCBI GenBank Nucleotide #: NM_171825.2
• UniProt Accession #: Q9UQM7; Q9UL21; Q9Y2H4; Q9Y352
• Molecular Weight: 55,320 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type II subunit alpha isoform 2
• NCBI Official Synonym Full Names: calcium/calmodulin dependent protein kinase II alpha
• NCBI Official Symbol: CAMK2A
• NCBI Official Synonym Symbols: CAMKA; MRD53
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit alpha
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit alpha
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMK2A
• UniProt Synonym Gene Names: CAMKA; KIAA0968; CaM kinase II subunit alpha; CaMK-II subunit alpha

NCBI Description

The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]

Uniprot Description

CAMK2A: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: CAMK group; CAMK2 family; EC 2.7.11.17; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: cell junction; cytoplasm; cytosol; mitochondrion; neuron projection; nucleoplasm; nucleus; plasma membrane; postsynaptic density; presynaptic membrane

Molecular Function: ATP binding; calcium-dependent protein serine/threonine kinase activity; calmodulin binding; calmodulin-dependent protein kinase activity; glutamate receptor binding; identical protein binding; kinase activity; metal ion binding; protein binding; protein homodimerization activity; protein serine/threonine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: angiotensin-activated signaling pathway; calcium ion transport; cell differentiation; dendrite morphogenesis; G1/S transition of mitotic cell cycle; MAPK cascade; nervous system development; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of calcium ion transport; positive regulation of cardiac muscle cell apoptotic process; positive regulation of NF-kappaB transcription factor activity; protein amino acid phosphorylation; protein autophosphorylation; regulation of cellular response to heat; regulation of mitochondrial membrane permeability involved in apoptotic process; regulation of neuronal synaptic plasticity; regulation of neurotransmitter secretion; Wnt receptor signaling pathway, calcium modulating pathway

Disease: Mental Retardation, Autosomal Dominant 53