Rabbit anti-Human, Mouse BMX Polyclonal Antibody | anti-BMX antibody

BMX, ID (Cytoplasmic Tyrosine-protein Kinase BMX, Bone Marrow TYrosine Kinase Gene In Chromosome X Protein, Epithelial And Endothelial Tyrosine Kinase, ETK, NTK38 (Biotin)

Cat. Num. AAA6465452

• Gene Names: BMX; ETK; PSCTK2; PSCTK3
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: BMX; Polyclonal Antibody; ID (Cytoplasmic Tyrosine-protein Kinase BMX; Bone Marrow TYrosine Kinase Gene In Chromosome X Protein; Epithelial And Endothelial Tyrosine Kinase; ETK; NTK38 (Biotin); anti-BMX antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BMX. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-BMX antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 212-242 in the central region of human BMX.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detecting BMX in mouse heart tissue lysate usingMBS627433.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS627433, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of MBS627433 for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-BMX antibody

Antibodies; Protein Kinases

References

1. Pan, S., et al., Mol. Cell. Biol. 22(21):7512-7523 (2002). 2. Qiu, Y., et al., Proc. Natl. Acad. Sci. USA 95(7):3644-3649 (1998). 3. Tamagnone, L., et al., Oncogene 9(12):3683-3688 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 4502435
• NCBI GeneID: 660
• NCBI Accession #: NP_001712.1
• NCBI GenBank Nucleotide #: NM_001721.6
• UniProt Accession #: P51813; O60564; Q12871; A6NIH9
• Molecular Weight: 78,011 Da
• NCBI Official Full Name: cytoplasmic tyrosine-protein kinase BMX
• NCBI Official Synonym Full Names: BMX non-receptor tyrosine kinase
• NCBI Official Symbol: BMX
• NCBI Official Synonym Symbols: ETK; PSCTK2; PSCTK3
• NCBI Protein Information: cytoplasmic tyrosine-protein kinase BMX; NTK38 tyrosine kinase; BTK-like on X chromosome; Etk/Bmx cytosolic tyrosine kinase; epithelial and endothelial tyrosine kinase; bone marrow tyrosine kinase gene in chromosome X protein
• UniProt Protein Name: Cytoplasmic tyrosine-protein kinase BMX
• Protein Family: Cytoplasmic tyrosine-protein kinase
• UniProt Gene Name: BMX
• UniProt Synonym Gene Names: ETK
• UniProt Entry Name: BMX_HUMAN

NCBI Description

This gene encodes a non-receptor tyrosine kinase belonging to the Tec kinase family. The protein contains a PH-like domain, which mediates membrane targeting by binding to phosphatidylinositol 3,4,5-triphosphate (PIP3), and a SH2 domain that binds to tyrosine-phosphorylated proteins and functions in signal transduction. The protein is implicated in several signal transduction pathways including the Stat pathway, and regulates differentiation and tumorigenicity of several types of cancer cells. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Sep 2009]

Uniprot Description

Etk: a tyrosine kinase of the Tec family. Activity is required for IL6-induced differentiation. May play a role in the growth and differentiation of hematopoietic cells. May be involved in signal transduction in endocardial and arterial endothelial cells.

Protein type: EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; TK group; Tec family

Chromosomal Location of Human Ortholog: Xp22.2

Cellular Component: extrinsic to internal side of plasma membrane; cytosol

Molecular Function: signal transducer activity; protein binding; protein-tyrosine kinase activity; metal ion binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: B cell receptor signaling pathway; adaptive immune response; apoptosis; protein amino acid autophosphorylation; innate immune response; mesoderm development; cell adhesion; signal transduction; cell differentiation; T cell receptor signaling pathway; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation; cell structure disassembly during apoptosis