Typical Testing Data/Standard Curve (for reference only)
Human CAMK2alpha (Calcium/Calmodulin-Dependent Protein Kinase II Alpha) CLIA Kit | CAMK2a clia kit
Human CAMK2alpha (Calcium/Calmodulin-Dependent Protein Kinase II Alpha) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to CAMK2alpha. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CAMK2alpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CAMK2alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of CAMK2alpha. You can calculate the concentration of CAMK2alpha in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]
Uniprot Description
CAMK2A: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.17; CAMK group; CAMK2 family
Chromosomal Location of Human Ortholog: 5q32
Cellular Component: nucleoplasm; presynaptic membrane; mitochondrion; plasma membrane; nucleus; cytosol; cell junction
Molecular Function: calmodulin binding; protein serine/threonine kinase activity; protein binding; glutamate receptor binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; kinase activity; ATP binding
Biological Process: epidermal growth factor receptor signaling pathway; axon guidance; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; activation of MAPKK activity; protein amino acid autophosphorylation; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; regulation of neurotransmitter secretion; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; synaptic transmission; peptidyl-serine phosphorylation; calcium ion transport; small GTPase mediated signal transduction; Ras protein signal transduction; insulin receptor signaling pathway; innate immune response; positive regulation of calcium ion transport; vascular endothelial growth factor receptor signaling pathway; G1/S transition of mitotic cell cycle; regulation of neuronal synaptic plasticity
Research Articles on CAMK2a
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Product Notes
The Human CAMK2a camk2a (Catalog #AAA2532542) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2532542 CLIA Kit recognizes Human CAMK2a. It is sometimes possible for the material contained within the vial of "CAMK2alpha (Calcium/Calmodulin-Dependent Protein Kinase II Alpha), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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