Human Calcium/Calmodulin-Dependent Protein Kinase II Alpha ELISA Kit | CAMK2alpha elisa kit

Human Calcium/Calmodulin-Dependent Protein Kinase II Alpha ELISA Kit

Cat. Num. AAA760890

• Gene Names: CAMK2A; CAMKA
• Reactivity: Human
• Synonyms: Calcium/Calmodulin-Dependent Protein Kinase II Alpha; Human Calcium/Calmodulin-Dependent Protein Kinase II Alpha ELISA Kit; CAMK2alpha/CAMKA ; CAMK2alpha elisa kit

• Reactivity: Human
• Sequence Length: 478

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CAMK2alpha elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human CAMK2alpha antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CAMK2alpha antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CAMK2alpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human CAMK2alpha can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 25952118
• NCBI GeneID: 815
• NCBI Accession #: NP_741960.1
• NCBI GenBank Nucleotide #: NM_171825.2
• Molecular Weight: 55,320 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type II subunit alpha isoform 2
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II alpha
• NCBI Official Symbol: CAMK2A
• NCBI Official Synonym Symbols: CAMKA
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit alpha
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit alpha
• UniProt Gene Name: CAMK2A
• UniProt Synonym Gene Names: CAMKA; KIAA0968; CaM kinase II subunit alpha; CaMK-II subunit alpha
• UniProt Entry Name: KCC2A_HUMAN

NCBI Description

The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]

Uniprot Description

CAMK2A: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.17; Protein kinase, CAMK; CAMK group; CAMK2 family

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: cell junction; cytosol; mitochondrion; nucleoplasm; nucleus; plasma membrane; presynaptic membrane

Molecular Function: ATP binding; calmodulin binding; calmodulin-dependent protein kinase activity; glutamate receptor binding; kinase activity; protein binding; protein homodimerization activity; protein serine/threonine kinase activity

Biological Process: activation of MAPKK activity; activation of NF-kappaB transcription factor; axon guidance; calcium ion transport; cytokine and chemokine mediated signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; G1/S transition of mitotic cell cycle; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; peptidyl-serine phosphorylation; positive regulation of calcium ion transport; protein amino acid autophosphorylation; protein amino acid phosphorylation; Ras protein signal transduction; regulation of neuronal synaptic plasticity; regulation of neurotransmitter secretion; small GTPase mediated signal transduction; synaptic transmission; vascular endothelial growth factor receptor signaling pathway