Human Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2) ELISA Kit | CHRNb2 elisa kit

Human Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2) ELISA Kit

Cat. Num. AAA2703386

• Gene Names: CHRNB2; EFNL3; nAChRB2
• Reactivity: Human
• Synonyms: Cholinergic Receptor; Nicotinic; Beta 2 (CHRNb2); Human Cholinergic Receptor; Beta 2 (CHRNb2) ELISA Kit; CHR-NB2; CHRN-B2; EFNL3; NAChRB2; N-AChR-B2; N-AChRB2; Neuronal Acetylcholine Receptor Beta 2; CHRNb2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2).; No significant cross-reactivity or interference between Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2) and analogues was observed.
• Sequence Length: 502

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHRNb2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119573577
• NCBI GeneID: 1141
• UniProt Accession #: P17787; Q9UEH9
• Molecular Weight: 57,019 Da
• NCBI Official Full Name: cholinergic receptor, nicotinic, beta 2 (neuronal)
• NCBI Official Synonym Full Names: cholinergic receptor nicotinic beta 2 subunit
• NCBI Official Symbol: CHRNB2
• NCBI Official Synonym Symbols: EFNL3; nAChRB2
• NCBI Protein Information: neuronal acetylcholine receptor subunit beta-2
• UniProt Protein Name: Neuronal acetylcholine receptor subunit beta-2
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNB2

NCBI Description

Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy. [provided by RefSeq, Jul 2008]

Uniprot Description

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodiun ions.