Human Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2) ELISA Kit | CHRNb2 elisa kit

Human Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2) ELISA Kit

Cat. Num. AAA2020303

• Gene Names: CHRNB2; EFNL3; nAChRB2
• Reactivity: Human
• Synonyms: Cholinergic Receptor; Nicotinic; Beta 2 (CHRNb2); Human Cholinergic Receptor; Beta 2 (CHRNb2) ELISA Kit; CHR-NB2; CHRN-B2; EFNL3; NAChRB2; N-AChR-B2; N-AChRB2; Neuronal Acetylcholine Receptor Beta 2; CHRNb2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2).; No significant cross-reactivity or interference between Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2) and analogues was observed.
• Sequence Length: 502

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHRNb2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CHRNb2 elisa kit

Signal transduction; Neuroscience; Developmental science; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 119573577
• NCBI GeneID: 1141
• UniProt Accession #: P17787; Q9UEH9
• Molecular Weight: 57,019 Da
• NCBI Official Full Name: cholinergic receptor, nicotinic, beta 2 (neuronal)
• NCBI Official Synonym Full Names: cholinergic receptor, nicotinic, beta 2 (neuronal)
• NCBI Official Symbol: CHRNB2
• NCBI Official Synonym Symbols: EFNL3; nAChRB2
• NCBI Protein Information: neuronal acetylcholine receptor subunit beta-2
• UniProt Protein Name: Neuronal acetylcholine receptor subunit beta-2
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNB2
• UniProt Entry Name: ACHB2_HUMAN

NCBI Description

Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy. [provided by RefSeq, Jul 2008]

Uniprot Description

nAChRB2: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRNB2 are the cause of nocturnal frontal lobe epilepsy type 3 (ENFL3). ENFL3 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta- 2/CHRNB2 sub-subfamily.

Protein type: Membrane protein, multi-pass; Receptor, misc.; Channel, cation; Membrane protein, integral; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; plasma membrane; integral to membrane; cell junction; external side of plasma membrane

Molecular Function: protein heterodimerization activity; acetylcholine receptor activity; drug binding; nicotinic acetylcholine-activated cation-selective channel activity; acetylcholine binding; ligand-gated ion channel activity

Biological Process: response to nicotine; positive regulation of dopamine secretion; protein heterooligomerization; regulation of dendrite morphogenesis; behavioral response to nicotine; locomotory behavior; regulation of circadian sleep/wake cycle, non-REM sleep; signal transduction; sensory perception of pain; regulation of dopamine secretion; synaptic transmission, cholinergic; synaptic transmission; regulation of synaptic transmission, dopaminergic; smooth muscle contraction; sensory perception of sound; visual perception; conditioned taste aversion; calcium ion transport; positive regulation of B cell proliferation; visual learning; synaptic transmission involved in micturition; associative learning; regulation of circadian sleep/wake cycle, REM sleep; vestibulocochlear nerve development; regulation of action potential; B cell activation; regulation of dopamine metabolic process; positive regulation of synaptic transmission, dopaminergic; central nervous system projection neuron axonogenesis; learning; negative regulation of action potential; optic nerve morphogenesis; social behavior; response to cocaine; memory; neurological system process; membrane depolarization; response to ethanol; regulation of synaptogenesis; response to hypoxia; ion transport; cognition; lateral geniculate nucleus development

Disease: Epilepsy, Nocturnal Frontal Lobe, 3