Mouse Estrogen Receptor Beta (ERb) ELISA Kit | ERb elisa kit

Mouse Estrogen Receptor Beta (ERb) ELISA Kit

Cat. Num. AAA2701802

• Gene Names: Esr2; Erb2; ERbeta; ER-beta
• Reactivity: Mouse
• Synonyms: Estrogen Receptor Beta (ERb); Mouse Estrogen Receptor Beta (ERb) ELISA Kit; ER-B; ESRB; ESTRB; NR3A2; NR3-A2; ESR2; Estrogen Receptor 2; Nuclear Receptor Subfamily 3; Group A; Member 2; ERb elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Estrogen Receptor Beta (ERb).; No significant cross-reactivity or interference between Estrogen Receptor Beta (ERb) and analogues was observed.
• Sequence Length: 485

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.142ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Related Product Information for ERb elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Estrogen Receptor Beta (ERb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Estrogen Receptor Beta (ERb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Estrogen Receptor Beta (ERb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen Receptor Beta (ERb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6978817
• NCBI GeneID: 25149
• NCBI Accession #: NP_036886.1
• NCBI GenBank Nucleotide #: NM_012754.1
• UniProt Accession #: Q62986; O35784; O35785; O55015; O55016; O70195; Q9R185
• Molecular Weight: 47,904 Da
• NCBI Official Full Name: estrogen receptor beta
• NCBI Official Synonym Full Names: estrogen receptor 2
• NCBI Official Symbol: Esr2
• NCBI Official Synonym Symbols: Erb2; ERbeta; ER-beta
• NCBI Protein Information: estrogen receptor beta
• UniProt Protein Name: Estrogen receptor beta
• UniProt Gene Name: Esr2
• UniProt Synonym Gene Names: Erbeta; Nr3a2; ER-beta

NCBI Description

binds estrogen and mediates transcriptional activation [RGD, Feb 2006]

Uniprot Description

Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform 3 and isoform 4 are unable to bind DNA and activate transcription due to the truncation of the DNA binding domain. Isoform 2 shows loss of ligand binding affinity and suppresses ER-alpha and ER-beta1 mediated transcriptional activation and may act as a dominant negative regulator of estrogen action.