Western Blot (WB)
(Western Blot: Sample: Recombinant ERb, Rat.)
Rabbit anti-Rat Estrogen Receptor Beta (ERb) Polyclonal Antibody | anti-ERb antibody
Polyclonal Antibody to Estrogen Receptor Beta (ERb)
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Recombinant ERb, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ERb, Rat.)
NCBI and Uniprot Product Information
NCBI Description
binds estrogen and mediates transcriptional activation [RGD, Feb 2006]
Uniprot Description
ER-beta: a nuclear hormone receptor and transcription factor. Binds and activated by estrogen. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Eight alternatively-spliced isoforms have been described. Isoform beta-cx lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.
Protein type: DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: 6q24
Cellular Component: cell soma; cilium; cytoplasm; mitochondrion; neuron projection; nucleus; perikaryon; perinuclear region of cytoplasm; plasma membrane
Molecular Function: DNA binding; DNA binding transcription factor activity; drug binding; enzyme binding; estrogen receptor activity; estrogen response element binding; hormone binding; peroxisome proliferator activated receptor binding; protein binding; sequence-specific DNA binding; steroid binding; steroid hormone receptor activity; zinc ion binding
Biological Process: amygdala development; behavioral fear response; brain development; cerebellum development; female gonad development; hormone-mediated apoptotic signaling pathway; hypothalamus development; intracellular estrogen receptor signaling pathway; learning and/or memory; male gonad development; negative regulation of behavior; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of smooth muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; neuron migration; ovarian follicle development; positive regulation of apoptosis; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; prostate gland development; regulation of cell proliferation; regulation of neuron apoptosis; regulation of transcription, DNA-templated; response to activity; response to drug; response to estradiol; response to estrogen; response to ethanol; response to genistein; response to hormone; response to insecticide; response to nutrient levels; response to organic cyclic compound; response to testosterone stimulus; response to water deprivation; Sertoli cell development; Sertoli cell proliferation; steroid hormone mediated signaling; steroid hormone receptor signaling pathway; transcription from RNA polymerase II promoter; transcription, DNA-dependent; uterus development; vagina development
Research Articles on ERb
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Product Notes
The ERb esr2 (Catalog #AAA2085968) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Estrogen Receptor Beta (ERb) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Estrogen Receptor Beta (ERb) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Researchers should empirically determine the suitability of the ERb esr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Estrogen Receptor Beta (ERb), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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