Porcine alpha-enolase (alphaENOL) ELISA Kit | alphaENOL elisa kit

Porcine alpha-enolase (alphaENOL) ELISA Kit

Cat. Num. AAA266509

• Gene Names: Eno1; Nne
• Reactivity: Porcine
• Synonyms: alpha-enolase (alphaENOL); Porcine alpha-enolase (alphaENOL) ELISA Kit; Porcine a-enolase (aENOL) ELISA Kit; alphaENOL elisa kit

• Reactivity: Porcine
• Sequence Length: 434

• Samples: Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant alphaENOL concentration
• Assay Type: Sandwich
• Detection Range: 10 ng/ml-0.156 ng/ml
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for alphaENOL elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine alphaENOL monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 158186649
• NCBI GeneID: 24333
• NCBI Accession #: NP_036686.2
• NCBI GenBank Nucleotide #: NM_012554.3
• Molecular Weight: 47,128 Da
• NCBI Official Full Name: alpha-enolase
• NCBI Official Synonym Full Names: enolase 1, (alpha)
• NCBI Official Symbol: Eno1
• NCBI Official Synonym Symbols: Nne
• NCBI Protein Information: alpha-enolase; alpha enolase; non-neural enolase; enolase 1, alpha non-neuron; 2-phospho-D-glycerate hydro-lyase
• UniProt Protein Name: Alpha-enolase
• UniProt Gene Name: Eno1
• UniProt Synonym Gene Names: Eno-1; NNE
• UniProt Entry Name: ENOA_RAT

Uniprot Description

ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Transcription, coactivator/corepressor; Lyase; EC 4.2.1.11

Cellular Component: extrinsic to plasma membrane; extracellular space; neuron projection; phosphopyruvate hydratase complex; membrane; cytoplasm; nucleus

Molecular Function: protein homodimerization activity; RNA binding; protein heterodimerization activity; magnesium ion binding; phosphopyruvate hydratase activity

Biological Process: glycolysis; in utero embryonic development; response to virus; negative regulation of cell growth; positive regulation of binding; negative regulation of transcription, DNA-dependent