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Typical Testing Data/Standard Curve (for reference only)

Bovine alpha-enolase (alphaENOL) ELISA Kit | alphaENOL elisa kit

Bovine alpha-enolase(alphaENOL) ELISA Kit

Gene Names
Eno1; Nne
Reactivity
Bovine
Synonyms
alpha-enolase (alphaENOL); Bovine alpha-enolase(alphaENOL) ELISA Kit; alphaENOL elisa kit
Ordering
For Research Use Only!
Reactivity
Bovine
Specificity
No cross-reaction with other factors.
Sequence Length
434
Samples
Bovine serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
10 ng/ml-0.156 ng/ml
Sensitivity
Up to 0.05 ng/ml
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for alphaENOL elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Bovine alphaENOL monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,128 Da
NCBI Official Full Name
alpha-enolase
NCBI Official Synonym Full Names
enolase 1
NCBI Official Symbol
Eno1
NCBI Official Synonym Symbols
Nne
NCBI Protein Information
alpha-enolase
UniProt Protein Name
Alpha-enolase
UniProt Gene Name
Eno1
UniProt Synonym Gene Names
Eno-1; NNE
UniProt Entry Name
ENOA_RAT

Uniprot Description

ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Lyase; Transcription, coactivator/corepressor

Cellular Component: cytoplasm; extracellular space; extrinsic to plasma membrane; membrane; myelin sheath; neuron projection; nucleus

Molecular Function: GTPase binding; heat shock protein binding; phosphopyruvate hydratase activity; protein binding; protein heterodimerization activity; protein homodimerization activity; RNA binding

Biological Process: in utero embryonic development; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; positive regulation of binding; response to virus

Research Articles on alphaENOL

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Product Notes

The Bovine alphaENOL eno1 (Catalog #AAA2608653) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2608653 ELISA Kit recognizes Bovine alphaENOL. It is sometimes possible for the material contained within the vial of "alpha-enolase (alphaENOL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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