BCAM recombinant protein
Recombinant Human BCAM Protein (C-His)
Purity
>95% as determined by reducing SDS-PAGE.
Synonyms
BCAM; Recombinant Human BCAM Protein (C-His); Basal cell adhesion molecule; Auberger B antigen; B-CAM cell surface glycoprotein; F8/G253 antigen; Lutheran antigen; Lutheran blood group glycoprotein; CD239; LU; MSK19; BCAM recombinant protein
Host
Human Cells
Purity/Purification
>95% as determined by reducing SDS-PAGE.
Form/Format
Lyophilized from a 0.2 mum filtered solution of PBS, pH7.4.
Sequence
Glu32-Ala547
Sequence Length
628
Species
Human
Endotoxin
< 1.0 EU per mug as determined by the LAL method.
Fusion Tag
C-His
Preparation and Storage
Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.
Related Product Information for BCAM recombinant protein
Protein Construction: Recombinant Human BCAM is produced by our mammalian expression system and the target gene encoding Glu32-Ala547 is expressed with a 6His tag at the C-terminus.
Background: Basal cell adhesion molecule (BCAM, CD239) is an immunoglobulin superfamily protein that arises from alternate splicing of the Lutheran blood group molecule (Lu). The ECD of human BCAM contains two Ig-like V-type domains and three Ig-like C2-type domains. It shares 73% aa sequence identity with the ECDs of mouse and rat BCAM. BCAM is widely expressed in epithelial and endothelial tissues including in the vasculature, kidney glomerulus, small intestine, colon, hair follicle outer root sheath, and basal keratinocytes of the skin during inflammation. BCAM is also expressed on vascular and visceral smooth muscle cells and at the neuromuscular junction of skeletal muscle. BCAM is upregulated on carcinomas, particularly ovarian, sarcomas, astrocytomas, and melanomas. It may mediate intracellular signaling. It cooperates with Integrins beta1 and alphaVbeta3 as an adhesion receptor for Laminins which contain the alpha5 chain. The Lutheran isoform is aberrantly phosphorylated in erythroid disorders and can enhance Lamininmediated adhesion of erythrocytes to vascular endothelial cells.
Background: Basal cell adhesion molecule (BCAM, CD239) is an immunoglobulin superfamily protein that arises from alternate splicing of the Lutheran blood group molecule (Lu). The ECD of human BCAM contains two Ig-like V-type domains and three Ig-like C2-type domains. It shares 73% aa sequence identity with the ECDs of mouse and rat BCAM. BCAM is widely expressed in epithelial and endothelial tissues including in the vasculature, kidney glomerulus, small intestine, colon, hair follicle outer root sheath, and basal keratinocytes of the skin during inflammation. BCAM is also expressed on vascular and visceral smooth muscle cells and at the neuromuscular junction of skeletal muscle. BCAM is upregulated on carcinomas, particularly ovarian, sarcomas, astrocytomas, and melanomas. It may mediate intracellular signaling. It cooperates with Integrins beta1 and alphaVbeta3 as an adhesion receptor for Laminins which contain the alpha5 chain. The Lutheran isoform is aberrantly phosphorylated in erythroid disorders and can enhance Lamininmediated adhesion of erythrocytes to vascular endothelial cells.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
Molecular Mass: 57kDa
Actual Protein Molecular Mass: 72-85kDa
Actual Protein Molecular Mass: 72-85kDa
NCBI Official Full Name
Basal cell adhesion molecule
UniProt Protein Name
Basal cell adhesion molecule
Protein Family
UniProt Gene Name
BCAM
UniProt Synonym Gene Names
LU; MSK19
Uniprot Description
Laminin alpha-5 receptor. May mediate intracellular signaling.
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Product Notes
The BCAM bcam (Catalog #AAA2557363) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Glu32-Ala5 47. It is sometimes possible for the material contained within the vial of "BCAM, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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