Human BCAM ELISA Kit | BCAM elisa kit

Human BCAM PicoKine ELISA Kit

Cat. Num. AAA178575

• Gene Names: BCAM; AU; LU; CD239; MSK19
• Reactivity: Human
• Synonyms: BCAM; Human BCAM PicoKine ELISA Kit; AU; Bcam; CD239; CD239 antigen; F8/G253 antigen; LU; Lutheran; MSK19; P50895; Basal cell adhesion molecule; basal cell adhesion molecule (Lutheran blood group); BCAM elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human BCAM
• Sequence Length: 588

• Samples: Cell culture supernates, serum and plasma(heparin, EDTA).
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <15pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).

Typical Testing Data/Standard Curve (for reference only)

(Human BCAM PicoKine ELISA Kit standard curve )

Related Product Information for BCAM elisa kit

Principle of the Assay: Human BCAM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BCAM has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: E32-A547) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BCAM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin- Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human BCAM amount of sample captured in plate.

Background/Introduction: Basal cell adhesion molecule is a protein that in humans is encoded by the BCAM gene. The BCAM gene encodes a glycoprotein expressed on cell surfaces. It is a member of the immunoglobulin superfamily of receptors and cell adhesion molecules. When expressed on erythrocytes, BCAM constitutes the Lutheran (Lu) and Auberger (Au) blood group antigens. And this protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 61742797
• NCBI GeneID: 4059
• NCBI Accession #: NP_001013275.1
• NCBI GenBank Nucleotide #: NM_001013257.2
• UniProt Accession #: P50895; Q86VC7; A8MYF9; A9YWT5; A9YWT6
• Molecular Weight: 67,405 Da
• NCBI Official Full Name: basal cell adhesion molecule isoform 2
• NCBI Official Synonym Full Names: basal cell adhesion molecule (Lutheran blood group)
• NCBI Official Symbol: BCAM
• NCBI Official Synonym Symbols: AU; LU; CD239; MSK19
• NCBI Protein Information: basal cell adhesion molecule
• UniProt Protein Name: Basal cell adhesion molecule
• Protein Family: Basal cell adhesion molecule
• UniProt Gene Name: BCAM
• UniProt Synonym Gene Names: LU; MSK19
• UniProt Entry Name: BCAM_HUMAN

NCBI Description

This gene encodes Lutheran blood group glycoprotein, a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

Lu: Laminin alpha-5 receptor. May mediate intracellular signaling.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: external side of plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function: laminin binding; laminin receptor activity; protein binding; protein C-terminus binding; transmembrane receptor activity

Biological Process: cell adhesion; cell-matrix adhesion; signal transduction

Disease: Blood Group--lutheran System; Lutheran Null