7-AAD Viability Staining Solution | 7-AAD staining solution

7-AAD Viability Staining Solution

Cat. Num. AAA2557013

• Applications: Apoptosis
• Synonyms: 7-AAD Viability; 7-AAD Viability Staining Solution; 7-AAD staining solution

• Applicable Applications for 7-AAD staining solution: Apoptosis
• Related Products: Annexin V-FITC Reagent (MBS2557006); Annexin V-AF647 Reagent (MBS2557007); Annexin V-PE Reagent (MBS2557008); Annexin V-APC Reagent (MBS2557009); Annexin V-EGFP Reagent (MBS2557010); Annexin V Binding Buffer(10 ×) (MBS2557011); Propidium Iodide (PI) Staining Solution (MBS2557012); Annexin V-FITC / PI Apoptosis Detection Kit (MBS2557014); Annexin V-FITC / 7-AAD Apoptosis Detection Kit (MBS2557015); Annexin V-AF647 / PI Apoptosis Detection Kit (MBS2557016); Annexin V-AF647 / 7-AAD Apoptosis Detection Kit (MBS2557017); Annexin V-PE / 7-AAD Apoptosis Detection Kit (MBS2557018); Annexin V-APC / PI Apoptosis Detection Kit (MBS2557019); Annexin V-APC / 7-AAD Apoptosis Detection Kit (MBS2557020); Annexin V-EGFP / PI Apoptosis Detection Kit (MBS2557021); Annexin V-EGFP / 7-AAD Apoptosis Detection Kit (MBS2557022)
• Staining Procedure: 1. Induce apoptosis of suspension cells with reagents of interest. Collect cell cultures and centrifuge at 300 g for 5 min, discard the supernatant. Add appropriate PBS to wash the cells, resuspend gently and count the cells.; Note: This product is only validated in suspension cells. Good cell viability is the key to the experiment. When the adherent cells are used for apoptotic detection, treatments like digestion may increase the ratio of necrotic or apoptotic cells and cause uncontrollable effects on the experimental results. Please be aware!; 2. Split the cell suspension into tubes, 1-5 × 10^5 cells for each. Centrifuge at 300 g for 5 min, discard the supernatant. Add appropriate PBS to wash the cells and discard the supernatant. Add 500 uL of 1 × Annexin V Binding Buffer[#Cat: MBS2557011 ] to resuspend the cells.; 3. Add 5 ul of Annexin V-APC[#Cat: MBS2557009 ] and 5 ul of 7-AAD Viability Staining Solution to each tube.; 4. Gently vortex the cells and incubate at room temperature for 15-20 min in the dark.; 5. Analyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.
• Preparation and Storage: Store at -20°C for up to one year in dark. 7-AAD Viability Staining Solution should be spilt into small tubes. ; Cautions:; 2. Detect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.; 1. For maximal assay performance, this reagent should be used within 3-6 months. Avoid freeze / thaw cycles.; 3. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.; 4. For your safety and health, please wear the lab coat and disposable gloves before the experiments.

Test Data

(Jurkat cells were treated with 1uM Camptothecin and detected by this reagent and Annexin V-APC.)

Related Product Information for 7-AAD staining solution

7-AAD Viability Staining Solution is developed to identify apoptotic and necrotic cells. 7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to the loss of integrity of membrane, 7-AAD can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using 7-AAD and Annexin V.

Product Categories/Family for 7-AAD staining solution

Apoptosis Detection Kit