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Western Blot (WB)
(Western Blot analysis of Human fetal brain tissue using CDC42/RHO/RAC Polyclonal Antibody at dilution of 1:200)
Actin Nucleation and Polymerization Antibody Kit
Actin Nucleation and Polymerization Antibody Sampler Kit
Applications
Western Blot
Synonyms
Actin Nucleation and Polymerization; Actin Nucleation and Polymerization Antibody Sampler Kit; Actin Nucleation and Polymerization antibody kit
Applicable Applications for Actin Nucleation and Polymerization antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of Human fetal brain tissue using CDC42/RHO/RAC Polyclonal Antibody at dilution of 1:200)
Western Blot (WB)
(Western Blot analysis of Human fetal brain tissue using CDC42/RHO/RAC Polyclonal Antibody at dilution of 1:200)
Western Blot (WB)
(Western Blot analysis of 293T cells with WAVE2 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of 293T cells with WAVE2 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of Human seminoma tissue, hela and A549 cell using PFN1 Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of Human seminoma tissue, hela and A549 cell using PFN1 Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of K562 cells with Arp3 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of K562 cells with Arp3 Polyclonal Antibody.)
Related Product Information for Actin Nucleation and Polymerization antibody kit
Background: Actin nucleation is the process of forming new actin filaments and is necessary to stimulate actin polymerization. Actin polymerization is vital for cell motility, cell division, and cell adhesion. Rac and Cdc42, members of the Rho-GTPase family, play key roles in actin dynamics, membrane trafficking, transcriptional regulation, cell growth, and development. GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the intrinsic GTPase activity or Rac/Cdc42, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through binding of RhoGDI, a guanine dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state. WAVE-2 is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in the brain. The highly conserved ARP2/3 complex is an important actin nucleation protein complex consisting of ARP2, ARP3, and ARPC1-5. The ARP2/3 complex promotes branching of existing actin filaments and formation of daughter filaments following activation by nucleation-promoting factors, such as WASP/WAVE or cortactin. Profilins are conserved actin binding proteins that affect the rate of actin polymerization by binding actin monomers and promoting exchange of ADP for ATP. Profilins bind to proteins involved in the regulation of actin dynamics including paladin, dynamin-I, VASP, and N-WASP.
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Product Notes
The Actin Nucleation and Polymerization (Catalog #AAA2549990) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Actin Nucleation and Polymerization can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Actin Nucleation and Polymerization for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin Nucleation and Polymerization, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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