SDS-PAGE
KIRREL3 / NEPH2 recombinant protein
Recombinant Mouse KIRREL3 / NEPH2 Protein (His tag)
Gene Names
Kirrel3; SST4; NEPH2; mKIAA1867; 1500010O20Rik; 2900036G11Rik
Purity
> 96 % as determined by SDS-PAGE
Synonyms
KIRREL3 / NEPH2; Recombinant Mouse KIRREL3 / NEPH2 Protein (His tag); 1500010O20Rik; 2900036G11Rik; NEPH2; SST4; mKIAA1867; KIRREL3 / NEPH2 recombinant protein
Host
Human Cells
Purity/Purification
> 96 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
778
Application Notes
The secreted recombinant mouse KIRREL3 consists of 525 amino acids (pro form) and has a calculated molecular mass of 57.5 kDa. The apparent molecular mass of the recombinant mouse KIRREL3 is approximately 65 kDa in SDS-PAGE under reducing conditions.
Predicted N Terminal
Leu 22
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for KIRREL3 / NEPH2 recombinant protein
Background: Kin of IRRE-like protein 3 (KIRREL3) also known as nin of irregular chiasm-like protein 3 or nephrin-like protein 2 (NEPH2) is a member of the nephrin-like protein family of transmembrane proteins, which includes NEPH1 (KIRREL) and NEPH3 (KIRREL2). KIRREL3/NEPH2 is expressed in fetalv and adult brain, and also in podocytes of kidney glomeruli. The cytoplasmic domains of KIRREL3/NEPH2 interact with the C-terminus of podocin, also expressed in the podocytes, cells involved in ensuring size- and charge-selective ultrafiltration. Mutations in KIRREL3/NEPH2 are associated with mental retardation autosomal dominant type 4. KIRREL3/NEPH2 expression is turned on in migrating nucleogenesis of the pontine nucleus (PN) neurons only after they enter the presumptive nuclear region. KIRREL3/NEPH2 knockdown disrupted the nuclear organization of PN presumably by changing the migratory behavior of PN neurons inside the nuclear region. Moreover, overexpression of the cytoplasmic region of KIRREL3, which can sequester intracellular signaling of endogenous KIRREL3, resulted in similar phenotype s. Overall, these results suggest KIRREL3 is involved in the nucleogenesis of the PN through the control of neuronal migration inside the nucleus.
Description: A DNA sequence encoding the mouse KIRREL3 (Q8BR86-1) extracellular domain (Met 1-Ala 535) was expressed, with a C-terminal polyhistidine tag.
Description: A DNA sequence encoding the mouse KIRREL3 (Q8BR86-1) extracellular domain (Met 1-Ala 535) was expressed, with a C-terminal polyhistidine tag.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,287 Da
NCBI Official Full Name
kin of IRRE-like protein 3 isoform A
NCBI Official Synonym Full Names
kin of IRRE like 3 (Drosophila)
NCBI Official Symbol
Kirrel3
NCBI Official Synonym Symbols
SST4; NEPH2; mKIAA1867; 1500010O20Rik; 2900036G11Rik
NCBI Protein Information
kin of IRRE-like protein 3
UniProt Protein Name
Kin of IRRE-like protein 3
UniProt Gene Name
Kirrel3
Uniprot Description
KIRREL3: Could be involved in the hematopoietic supportive capacity of stroma cells. A chromosomal aberration involving KIRREL3 and CDH15 is found in a patient with severe mental retardation and dysmorphic facial features. Translocation t(11;16)(q24.2;q24). Defects in KIRREL3 are the cause of mental retardation autosomal dominant type 4 (MRD4). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Belongs to the immunoglobulin superfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 9|9 A4
Cellular Component: axon; dendrite; dendritic shaft; extracellular region; integral to membrane; plasma membrane
Molecular Function: protein binding
Biological Process: hemopoiesis; hippocampus development; homophilic cell adhesion; neurite morphogenesis; neuron migration; pontine nucleus development; synaptogenesis
Research Articles on KIRREL3 / NEPH2
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Product Notes
The KIRREL3 / NEPH2 kirrel3 (Catalog #AAA2547025) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The secreted recombinant mouse KIRREL3 consists of 525 amino acids (pro form) and has a calculated molecular mass of 57.5 kDa. The apparent molecular mass of the recombinant mouse KIRREL3 is approximately 65 kDa in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the KIRREL3 / NEPH2 kirrel3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIRREL3 / NEPH2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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